Background and Aims: Recently, a novel DNA virus was isolated from the serum of a patient with post-transfusion non A-G hepatitis and named TT virus. The aim of this study was to determine the prevalence TT virus among cirrhotic patients due to hepatitis B & C in infection Ahwaz. Methods: The prevalence of TTV infection was studied in 41 patients with liver cirrhosis. TTV DNA was detected ...

Grapevine (Vitis vinifera L.) is one of important economic fruit crops found in Salahaldin province \ Iraq. To examine the Molecular characterization and genetic relationships among nine grape varieties by using random amplified polymorphic DNA (RAPD) marker. Fifteen RAPD primers produced band (71, 56%), while two yielded monomorphic bands only. The size fragment ranged between 250-2700 bp, wit...

DNA methylation is emerging as an attractive marker providing investigative leads to solve crimes in forensic genetics. The identification of body fluids that utilizes tissue-specific DNA methylation can contribute to solving crimes by predicting activity related to the evidence material. The age estimation based on DNA methylation is expected to reduce the number of potential suspects, when th...

The article discusses the relevance of determining DNA double-strand breaks (DSBs) using analysis γ-H2AX foci as a marker instability in various conditions. issues formation DSBs and peculiarities their detection tissues are highlighted. Changes intensity during use radiological diagnostic methods, stress, increased physical exertion, some oncological rheumatic diseases, well dynamics repair on...

Serving as a DNA molecular weight standard, the DNA ladder has been widely used in molecular biology applications. We developed a simple method for the preparation of a DNA marker, which involves designing primers to amplify 100- to 1000-bp DNA fragments using lambda DNA as a template for polymerase chain reaction, followed by extraction with phenol/chloroform, precipitation with ethanol and mi...

DNA methylation, an epigenetic mechanism is claimed to play essential roles in development, aging and disease over the past few decades. Cytosines (C) were known to exist in two functional states: unmethylated or methylated (5mC) in the mammalian genome for a very long time. However, the mechanisms controlling 5mC dynamics remain undefined. Recent studies of genomic DNA on human and mouse brain...

Genetic mapping with DNA markers has become an essential tool for revealing the genetic basis of both qualitative and quantitative traits in crop plants. DNA markers have special advantages over morphological traits in genetic analysis because they are normally polymorphic, have no pleiotropic or epistatic effects, employ non-destructive methods requiring very small amounts of plant materials a...