Doubled haploid (DH) populations are useful to scientists and breeders in both crop improvement and basic research. Current methods of producing DHs usually need in vitro culture for extracting haploids and chemical treatment for chromosome doubling. This report describes a simple method for synthesizing DHs (SynDH) especially for allopolyploid species by utilizing meiotic restitution genes. Th...

in order to locate the qtls for plant height, peduncle length, and heading date, a set of 107 wheat doubled haploid (dh) lines derived from the cross fukuho-komugi× oligoculm was grown during the growing seasons of 2004 and 2005. a total of 36 qtls were identified based on composite interval mapping (cim) approach. all detected plant height qtl’s were stable over the two years. qtls located nea...

Landraces are valuable genetic resources for broadening the genetic base of elite germplasm in maize. Extensive exploitation of landraces has been hampered by their genetic heterogeneity and heavy genetic load. These limitations may be overcome by the in-vivo doubled haploid (DH) technique. A set of 132 DH lines derived from three European landraces and 106 elite flint (EF) lines were genotyped...

Thousands of landraces are stored in seed banks as "gold reserves" for future use in plant breeding. In many crops, their utilization is hampered because they represent heterogeneous populations of heterozygous genotypes, which harbor a high genetic load. We show, with high-density genotyping in five landraces of maize, that libraries of doubled-haploid (DH) lines capture the allelic diversity ...

Production of pure lines is an important step in biological studies and breeding of many crop plants. The major types of pure lines for biological studies and breeding include doubled haploid (DH) lines, recombinant inbred lines (RILs), and near isogenic lines (NILs). DH lines can be produced through microspore and megaspore culture followed by chromosome doubling while RILs and NILs can be pro...

Contrast between marker-assisted backcross (MABC) and doubled haploid (DH) methods in transferring genes for resistance to maize lethal necrosis (MLN) (Zea mays L.) is not well understood. The MLN caused by co-infection of plant chlorotic mottle virus sugarcane mosaic virus. Two panels consisting four BC3F2 six DH populations, separately developed through selection from crosses susceptible CIMM...

Doubled haploid (DH) technology has been used in breeding programs for several decades and is currently the method of choice in a number of crop species, including barley (Hordeum vulgare L.), rapeseed (Brassica napus L.), maize (Zea mays L.), and wheat (Triticum aestivum L.). In this study we investigated via computer simulation the benefit of using DHs compared with the conventional wheat bre...

Camelina (Camelina sativa (L.) Crantz) is an emerging industrial crop from the Brassicaceae family, with its seed oil and cake being used for food, feed, fuel applications. In this study, relationships between economically important agronomic traits including yield (SY), days to maturity (DM), 1000-seed weight (TSW), protein content (PC), (OC), fatty acid composition in 136 doubled haploid (DH)...

Improvement in de novo assembly of large genomes is still to be desired. Here, we improved draft genome sequence quality by employing doubled-haploid individuals. We sequenced wildtype and doubled-haploid Takifugu rubripes genomes, under the same conditions, using the Illumina platform and assembled contigs with SOAPdenovo2. We observed 5.4-fold and 2.6-fold improvement in the sizes of the N50 ...