Considerable progress has been accomplished in the cellular and molecular biology of Brassica species in the past few years. Plant regeneration has been increasingly optimized via organogenesis and somatic embryogenesis using various explants; with tissue culture improvements focusing on factors such as age of the explant, genotype, and media additives. The production of haploids and doubled ha...

Doubled haploid plants are invaluable breeding tools but many crop species are recalcitrant to available haploid induction techniques. To test if haploid inducer lines can be engineered into crops, CENH3 (-∕-) and CENH3:RNAi lines were complemented by AcGREEN-tailswap-CENH3 or AcGREEN-CENH3 transgenes. Haploid induction rates were determined following testcrosses to wild-type plants after indep...

Doubled haploid (DH) populations are useful to scientists and breeders in both crop improvement and basic research. Current methods of producing DHs usually need in vitro culture for extracting haploids and chemical treatment for chromosome doubling. This report describes a simple method for synthesizing DHs (SynDH) especially for allopolyploid species by utilizing meiotic restitution genes. Th...

Doubled haploids (DH) have become a powerful tool to assist in different basic research studies, and also applied research. The principal (but not the only) routine use of DH by breeding companies is produce pure lines for hybrid seed production crop species. Several decades after discovery haploid inducer maize anther culture as method plants from pollen precursors, biotechnological revolution...

Albinism in plants is characterized by partial or complete loss of chlorophyll pigments and incomplete differentiation chloroplast membranes. This a very common problem encountered interspecific crosses tissue culture experiments including anther generation doubled haploids. impairs photosynthesis the eventually die at young stage without reaching maturity. Environmental conditions such as ligh...

The aim of the study was to development rice microspores in anther culture vitro , structure androclinic callus identify cyto-embryological features formation morphogenic structures culture, obtain doubled haploids ( Oryza sativa L .) and accelerate valuable breeding material with desired properties. Within framework this study, results a cyto-histological haploid androgenesis were obtained, wh...

Thielaviopsis basicola and Tomato spotted wilt virus (TSWV) are the most important problems in a moderate climate zone. Previously obtained doubled haploids (DH) of F1 hybrids of the flue-cured line WGL3 resistant to Th. basicola and the dark-cured line PW-834 carrying RTSW-al gene provided the research material. Biological tests and SCAR markers linked with TSWV were applied to confirm resista...