A method is described for the determination of thyroxine binding globulin (TBG) in dried blood spotted on filter paper using reagents from a test kit for the measurement of TBG in plasma. By minor modifications of the recommended procedure it was possible to improve precision, sensitivity and tracer displacement. Appropriate TBG standard samples were prepared in 'artificial blood' consisting of...

Measurement of 17-hydroxyprogesterone (17-OHP) in daily dried blood spot profiles via radioimmunoassay is a convenient and accepted method for monitoring of glucocorticoid therapy in Congenital Adrenal Hyperplasia. Characteristics of this method that serve to limit its clinical usefulness include its lack of specificity for 17-OHP, and a relatively high limit of detection. Mass Spectroscopy is ...

A screeningprocedure is described which can be used to separate the great majority of normal personsfrom those with a lack of the enzyme galactose transferase. The method is designed to be used on dried blood spot eluates which remain after a phenylalanine determination has been performed. The optimum conditions for this test are described, and data are presented from a preliminary study of mor...

BACKGROUND Mucopolysaccharidosis type II (MPS II) is a lysosomal storage disorder related to a deficiency in the enzyme iduronate-2-sulfatase (IDS). Clinical trials of enzyme replacement therapy are in progress, but effective treatment will require screening assays to enable early detection and diagnosis of MPS II. Our study evaluated the diagnostic accuracy of IDS protein and enzyme activity m...

We developed a blood spot test for syphilis antibody using enzyme-linked immunosorbent assay (ELISA) technology. Dried blood was eluted by buffered saline or, for a supplementary confirmatory test, by treponemal-antibody test diluent. Eluates were diluted in an absorption buffer (Calypte Biomedical, Berkeley, Calif.) and added to plate wells coated with cardiolipin antigen (ADI Diagnostics, Tor...

Background Effective health care delivery to the majority of perinatally exposed infants is hampered by lack of access to accurate HIV diagnosis in infancy. Polymerase chain reaction is the most sensitive test to diagnose HIV-1 infection in children born to HIV seropositive mothers. The purpose of this study was to assess the feasibility and accuracy of using dried blood spot (DBS) technology i...

We established the Bio-repository at the National Cancer Center Hospital in October 2002. The main purpose of this article is to show the importance and usefulness of a bio-repository of post-clinical test samples not only for translational cancer research but also for routine clinical oncology by introducing the experience of setting up such a facility. Our basic concept of a post-clinical tes...

The present article will provide an overview of use of dried blood sampling method for analysis of drug of abuse. Relatively short half life and instability in blood, calls for alternative sampling method for determination of drugs of abuse. Dried blood spot (DBS) method has many advantages over the conventional sampling methods. The available method for DBS sample collection, storage and trans...