نتایج جستجو برای: duplex rt pcr
تعداد نتایج: 214870 فیلتر نتایج به سال:
While meeting the pandemic demand of SARS-CoV-2 testing, clinical laboratories worldwide tend to adopt new test systems offering cost-effective and faster outcomes. However, reliability results has paramount importance in management such a health crisis. Therefore, this study sought determine accuracy from novel duplex Microchip RT-PCR system using patient saliva samples nasal swabs stabilized ...
The diagnostic work-up of acute promyelocytic leukemia (APL) includes the cytogenetic demonstration of the t(15;17) translocation and/or the PML-RARA chimeric transcript by RQ-PCR or RT-PCR. This latter assays provide suitable results in 3-6 hours. We describe here two new, rapid and specific assays that detect PML-RARA transcripts, based on the RT-QLAMP (Reverse Transcription-Quenching Loop-me...
PCR is the best method for the detection of enteric viruses present at low concentrations in environmental samples. However, some organic and inorganic compounds present in these samples can interfere in the reaction. Many of these substances are cytotoxic, too. The ZP60S filter membranes used in addition to fluorpentane treatment are quite efficient for virus concentration and simultaneous eli...
AIMS A novel molecular assay for the detection of foot-and-mouth disease virus (FMDV) was developed using linear-after-the-exponential polymerase chain reaction (LATE-PCR). METHODS AND RESULTS Pilot experiments using synthetic DNA targets demonstrated the ability of LATE-PCR to quantify initial target concentration through endpoint detection. A two-step protocol involving reverse transcriptio...
Tristeza and Huanglongbing are the most important diseases of citrus caused by Citrus tristeza virus (CTV) and ‘Candidatus Liberibacter’ species, respectively. A reverse transcription duplex polymerase chain reaction (RT-d-PCR) was developed to detect these pathogens simultaneously from infected citrus plants. Consistence of the technique was validated and specificity of the primers was confirm...
چکیده اسفناج (spinacia oleracea l.) یکی از مهمترین سبزیهای برگی است که به صورت تازه و یا فرآوری شده مصرف میشود و به دلیل داشتن خواص دارویی و مواد غذایی فراوان به صورت گسترده در دنیا کشت و مصرف میشود. گیاه اسفناج همواره تحت تاثیر بیمارگرهای مختلف قارچی، باکتریایی و ویروسی قرار میگیرد. در نمونهبرداریهای صورت گرفته از مزارع منطقه داوران شهرستان رفسنجان در سال1390، مجموعاً 177 نمونه از گیاها...
نمودار تعداد نتایج جستجو در هر سال
با کلیک روی نمودار نتایج را به سال انتشار فیلتر کنید