1. Insoluble salts of alklaine earth metals (e. g. calcium phosphate) dissolve in non-aqueous solvents containing EDTA salts of organic cations (e.g. tetraethylammonium). 2. Bone is efficiently demineralized in ethanolic trimethylammonium EDTA. Retention in the matrix of water-soluble materials (e.g. proteoglycan) is much better than in standard aqueous EDTA demineralization procedures.

The current study aims to understand the speciation and fate of Cu complexes in hydrothermally altered sediments from the Central Indian Ocean Basin and assess the probable impacts of deep-sea mining on speciation of Cu complexes and assess the Cu flux from this sediment to the water column in this area. This study suggests that most of the Cu was strongly associated with different binding site...

pies after ethanol/chloroform treatVu/us cells ment (for CUZnSOD assay) or for sam20 0.0053 0.0037 0.0027 0.0072 0.0070 0.0050 plea from tissues that contain a trace 0.0144 0.0121 0.0100 0.0179 0.0121 0.0102 amount of xanthine oxidase (EC 1.2.3.2) activity. Now, we have modifled the method by simply substituting 1 minolfL diethylenetriamine-pentaacetic acid (DETAPAC) for 0.1 mmoIJL EDTA and add...

Background Alzheimer’s disease (AD) is a progressive neurodegenerative disorder that threatens the population health of older adults. However, mechanisms how altered metabolism involves AD pathology are poorly understood. This study intends to provide novel insights into role metabolic alteration in and identify potential biomarkers for diagnosis Method The Gas chromatography-mass spectrometry ...

Three novel electron donor-acceptor conjugated polymers (P1-P3) bearing various imidazole pendants have been synthesized. Their excellent photophysical and electrochemical properties make them suitable transduction materials for chemosensing applications. Indeed, polymers P1-P3 have been found to show remarkable sensing capabilities towards H(+) and Fe(2+) in semi-aqueous solutions. Upon titrat...

Several types of microcarriers suitable for large-scale cultivation of mammalian cells are commercially available. However, many of these carriers have disadvantages, e.g., the need for enzymatic digestion for cell harvesting, size limitations and insufficient biocompatibility. These limitations have been overcome by the development of collagen-coated Ba(2+)-alginate microcarriers. Ba(2+)-algin...

The biological variation encountered in spot urine samples was assessed by collecting six sets of serial urine specimens from five men. The lead, coproporphyrin, and creatinine contents of each specimen were determined and the specific gravity was measured. It is found that as the mean concentration of the metabolite rises so the variability of the individual values increases. The scatter of th...

Simplified methods are described for the measurement of human plasma renin "concentration" (PRC) and "activity" (PRA) based on the denaturation of renin substrate in which separation and concentration steps are avoided and recovery of renin is complete. In the PRA method effective inhibition of angiotensinase is achieved by warming plasma at pH 4.5 with EDTA followed by dialysis to pH 7.5. Neit...

The plasma and kidney angiotensinases were compared in the rat . Effects of acid and alkali treatments and of enzyme inhibitors on the angiotensinase activities and the renin-angiotensionogen reaction were studied . Angiotensinase activity of the plasma was greatest near neutral pH at 37•Ž and was blocked almost completely by dialysis against disodium ethylenediaminetetraacetic acid (EDTA). The...