Normalization to reference genes is the most common method to avoid bias in real-time quantitative PCR (qPCR), which has been widely used for quantification of gene expression. Despite several studies on gene expression, Lilium, and particularly L. regale, has not been fully investigated regarding the evaluation of reference genes suitable for normalization. In this study, nine putative referen...

OBJECTIVE To evaluate the operation and surgical time of autotransplanted parathyroid in a single site comparing it to the implant performed in five and twenty locations. METHODS Patients who underwent total parotidectomy with auto implant (Ptx-AI) for secondary and tertiary hyperparathyroidism were evaluated in groups of 20 implant sites (A), 5 sites (B) and single site (C), compared as for ...

We study the existence of allocations indivisible goods that are envy-free up to one good (EF1), under additional constraint each bundle needs be connected in an underlying item graph. If graph is a path and utility functions monotonic over bundles, we show EF1 for at most four agents, EF2 any number agents; our proofs involve discrete analogues Stromquist's moving-knife protocol Su--Simmons ar...

Dječja reumatologija je relativno mlada i dinamična subspecijalistička struka koja se bavi dijagnostikom i intradisciplinarnim liječenjem sistemskih i mišićnokoštanih bolesti koje nastaju kao posljedica poremećaja u regulaciji imunološkog sustava, a najčešće razultiraju razvojem autoimunosti ili autoinfl amatorne reakcije te upalom. Postoje brojne razlike između djece i odraslih osoba koje bolu...

Trimethylation of Histone 3 lysine 27 (H3K27me3) is a chromatin modification that is associated with transcriptional repression (Cao et al., 2002; Sarma et al., 2008; Pengelly et al., 2013) [1], [2], [3]. In this article we performed anti-H3K27me3 Chromosomal Immunoprecipitation (ChIP-PCR), to detect the abundance of H3K27me3 marks on Ef1-alpha, cyp26A, HoxC10, HoxD10 and HoxD11 in the Xenopus ...

The Leptynia hispanica stick insect species complex includes bisexuals, triploid and tetraploid parthenogenetic populations, suggesting that polyploidy has played a central role in the evolution of this complex. An analysis of karyotype, mitochondrial DNA (cox2) and nuclear DNA (ef1-alpha) markers was carried out to clarify phylogenetic relationships and microevolutionary/phylogeographical patt...

Sorcin is an essential penta-EF hand calcium binding protein, able to confer the multi-drug resistance phenotype to drug-sensitive cancer cells and to reduce Endoplasmic Reticulum stress and cell death. Sorcin silencing blocks cell cycle progression in mitosis and induces cell death by triggering apoptosis. Sorcin participates in the modulation of calcium homeostasis and in calcium-dependent ce...

The quantitative real-time polymerase chain reaction (qRT-PCR) approach has become a widely used method to analyze expression patterns of target genes. The selection of an optimal reference gene is a prerequisite for the accurate normalization of gene expression in qRT-PCR. The present study constitutes the first systematic evaluation of potential reference genes in Rhododendron molle G. Don. E...

Telecommuting, or working part of the time from another location than the office, normally from home, has been tried by several organisations in the recent years. This has not always been a success. Still, many arguments in favour of telecommuting are forwarded by previous studies. This paper investigates the development of telecommuting in three organisations, and elaborates on mechanisms behi...