A number of epidermal growth factor (EGF)-resistant clones have been isolated from human epidermoid carcinoma A431 cells (I. King and A. C. Sartorelli, Biochem. Biophys. Res. Commun., 140: 837, 1986). These cells had a higher capacity to enter a pathway of terminal differentiation, as determined by their ability to form cornified envelopes. Thus, after 6 days in culture, 10% of parental A431 ce...

This paper demonstrates spontaneous transfer of epidermal growth factor (EGF) receptor mitogenicity from human A431 carcinoma cells to receptor negative mouse cells. The fact of transfer was documented by the demonstration in the recipient cell of normal EGF-binding affinity, covalent labeling of trans ferred receptor by 125I-EGF, and reactivity with specific antihuman receptor antibody. Phosph...

Binding of epidermal growth factor (EGF) to cell surface EGF receptors initiates the formation of the receptor homodimers that can be detected by covalent cross-linking in intact cells or in detergent-solubilized cell extracts. Low pH dissociation of EGF from surface receptors results in immediate monomerization of receptor dimers. Using chemical cross-linking during mild permeabilization or ce...

Luo, LuGuang, Naohiro Yano, and John Z. Q. Luo. The molecular mechanism of EGF receptor activation in pancreatic -cells by thyrotropin-releasing hormone. Am J Physiol Endocrinol Metab 290: E889–E899, 2006; doi:10.1152/ajpendo.00466.2005.—Thyrotropin-releasing hormone (TRH) and its receptor subtype TRH receptor-1 (TRHR1) are found in pancreatic -cells, and it has been shown that TRH might have p...

Overexpression and enhanced activation of the epidermal growth factor (EGF) receptor are frequent events in human cancers that correlate with poor prognosis. Anti-phosphotyrosine and anti-EGFr affinity chromatography, isotope-coded muLC-MS/MS, and immunoblot methods were combined to describe and measure signaling networks associated with EGF receptor activation and pharmacological inhibition. T...

This paper demonstrates spontaneous transfer of epidermal growth factor (EGF) receptor mitogenicity from human A431 carcinoma cells to receptor negative mouse cells. The fact of transfer was documented by the demonstration in the recipient cell of normal EGF-binding affinity, covalent labeling of transferred receptor by 125I-EGF, and reactivity with specific anti-human receptor antibody. Phosph...

We have identified physical and functional interactions between the epidermal growth factor (EGF) receptor and phospholipid scramblase 1 (PLSCR1), an endofacial plasma membrane protein proposed to affect phospholipid organization. PLSCR1, a palmitoylated protein, was found to partition with the EGF receptor in membrane lipid rafts. Cell stimulation with EGF transiently elevated Tyr-phosphorylat...

Previous studies have demonstrated that 17 beta-estradiol (E2) causes a 3-fold increase in epidermal growth factor (EGF) receptors in uterine membranes. We now report that the increase in uterine EGF receptor levels is due to an increase in the steady-state levels of EGF receptor mRNA. After a single E2 injection, EGF receptor mRNA levels, as determined by RNA blots, increase 3- to 4-fold betwe...

This paper describes studies on the migratory behavior of epidermal growth factor (EGF) receptor kinase using antibodies that are specific for either the kinase domain or the extracellular domain of the receptor. Antiserum was raised to a 42,000-D subfragment of EGF receptor, which was shown earlier to carry the kinase catalytic site but not the EGF-binding site. Another antiserum was raised to...

Upon activation, the epidermal growth factor (EGF) receptor becomes phosphorylated and triggers a vast signaling network that has profound effects on cell growth. The EGF receptor is observed to assemble into clusters after ligand binding and tyrosine kinase autophosphorylation, but the role of these assemblies in the receptor signaling pathway remains unclear. To address this question, we meas...