Nanoscale distance measurements by pulse dipolar Electron paramagnetic resonance (EPR) spectroscopy allow new insights into the structure and dynamics of complex biopolymers. EPR detection requires site directed spin labeling (SDSL) of biomolecule(s), which remained challenging for long RNAs up-to-date. Here, we demonstrate that novel complementary-addressed SDSL approach allows efficient spin ...

The purpose of this paper is to describe some of the areas where electron paramagnetic resonance (EPR) has provided unique information to MRI developments. The field of application mainly encompasses the EPR characterization of MRI paramagnetic contrast agents (gadolinium and manganese chelates, nitroxides) and superparamagnetic agents (iron oxide particles). The combined use of MRI and EPR has...

The role of enzyme engineering in biotechnology, biological and pharmaceutical process cannot be over emphasized. This study compared the adsorption of amylase and protease on to manganese ferrite (MnFe2O4). The metal ferrite was synthesized via a sol – gel technique and characterized with scanning electron microscopy (SEM), X-ray diffraction (XRD), Electron paramagnet...

Electron paramagnetic resonance (EPR) spectra of synthetic diamond microcrystals with NV (-) -centers have been studied. It is shown that under the conditions irradiation material light a xenon lamp at low temperatures ~ 100 K, intensities EPR signals corresponding to "forbidden" (Delta m s =2) and field allowed =1) transitions are amplified several times, while from nitrogen ...

Paramagnetic complexes of iron (III), manganese (II), and cobalt (II) protoporphyrins IX and apohemoproteins react with nitric oxide to form spectroscopically distinct compounds. Optical and electron paramagnetic resonance (EPR) studies suggest that a l-electron transfer between nitric oxides and metal ions which occurs in these reactions results in the formation of spin-paired complexes of the...

The pulsed electron paramagnetic resonance (EPR) methods of electron spin echo envelope modulation (ESEEM) and electron spin echo-electron nuclear double resonance (ESE-ENDOR) are used to investigate the structure of the Photosystem II oxygen-evolving complex (OEC), including the paramagnetic manganese cluster and its immediate surroundings. Recent unpublished results from the pulsed EPR labora...

Paramagnetic complexes of iron (III), manganese (II), and cobalt (II) protoporphyrins IX and apohemoproteins react with nitric oxide to form spectroscopically distinct compounds. Optical and electron paramagnetic resonance (EPR) studies suggest that a l-electron transfer between nitric oxides and metal ions which occurs in these reactions results in the formation of spin-paired complexes of the...

In this paper, the results of the synthesis and characterization of higher fullerene-based colloids is presented. The generation of singlet oxygen 1O2 (1Δg) by fullerene water-based colloids (nC60, nC70 and nC84) was investigated. It was found by electron paramagnetic resonance spectroscopy that the generation of singlet oxygen was the highest by the nC84 colloid. The amplitude of the electron ...

Paramagnetic complexes of iron (III), manganese (II), and cobalt (II) protoporphyrins IX and apohemoproteins react with nitric oxide to form spectroscopically distinct compounds. Optical and electron paramagnetic resonance (EPR) studies suggest that a l-electron transfer between nitric oxides and metal ions which occurs in these reactions results in the formation of spin-paired complexes of the...