In tear fluid, a large number of proteins can be detected with electrophoretic or immunologic techniques. The composition of serum proteins in tears resembles that of whole serum. By comparison of the protein patterns resulting from different sampling methods, it was shown that serum albumin is not present in the secretion of the lacrimal gland, but is mixed with the tear fluid in the conjuncti...

The relationship between proteins from blood serum and milk has been the subject of considerable study, with some contradictory results (1-5). This is especially true of albumins. Application of electrophoretic techniques to the analysis of whey proteins indicated the presence of a small component, consisting of approximately 3 to 5 per cent of the total protein, with the electrophoretic mobili...

BACKGROUND Serum free light chain assay (SFLCA) and κ/λ ratio, and protein electrophoretic methods are used in the diagnosis and monitoring of monoclonal gammopathies. METHODS Results for serum free light chains, serum and urine protein electrophoreses and immunofixation electrophoreses in 468 patients with a diagnosis of monoclonal gammopathy were compared. The results of the two methods wer...

Determination of the physiological electrophoretic patterns in animals is very useful for clinicians in diagnosing healthy and sick animals. The objective of this study was to investigate the serum protein electrophoretic pattern in cows, sheep, and goats in order to evaluate the differences in the size and number of protein fractions between the evaluated ruminant species. Ten adult multiparou...

UNLABELLED Recently developed proteomic technologies allow to profile thousands of proteins within a high-throughput approach towards biomarker discovery, although results are not as satisfactory as expected. In the present study we demonstrate that serum proteome denaturation is a key underestimated feature; in fact, a new differential denaturation protocol better discriminates serum proteins ...

Observations have been reported which suggest that altered electrophoretic patterns of the serum proteins may accompany pregnancies that terminate in the birth of abnormal offspring . The teratogenic disazo dye, trypan blue, has been shown to cause alterations in the proteins of the maternal serum of pregnant rabbits and in the protein composition of the 9-day rabbit embryo yolk sac fluid (1,2,...

In a consideration of the abnormalities of protein metabolism observed in multiple myeloma, a number of fundamental problems remain unsolved. To state just two of these, it remains to be determined (a) precisely what relationship the abnormal serum globulins of myeloma bear to normal serum gamma globulin, and (b) what is the nature of the possible inter-relationship between the abnormal serum p...

Capillary zone electrophoresis (CZE) of serum proteins is increasingly gaining impact in clinical laboratories. In this report, we evaluate automated capillary zone electrophoresis by Capillarys (Sebia, France). Within-run and between-run imprecision for the five electrophoretic fractions was <2% and <6%, respectively. Data obtained with Capillarys correlated with results obtained with agarose ...