Somatic embryogenesis was initiated in C. sativus L. from shoot meristems on LS medium containing BAP (2×10-5 M)+NAA (2×10-5 M). Various stages of somatic embryogenesis were observed in the same medium and the development was asynchronous. Somatic embryo development proceeded through well recognized sequences (globular to embryoids) with clearly discernible bipolar regions. Matured embryos coul...

microspore culture was applied to induce embryogenesis in roses (rosa hybrida) cv. hav1. important factors affecting viability of isolated microspores and formation of multi-cellular structures including isolation media (nln, ab, b) and induction media were studied. the most responsive developmental stage of microspores (late unicellular) was determined using dapi staining. the least infection ...

in this research, the isolated microspore culture system in 15 rose cultivars was tested. in the first experiment, the effect of rose cultivars on microspore viability and multicellular formation was examined. results showed that the most multicellular structures obtained in ‘apollo’, ‘amarosa’, ‘majic’, ‘candy’, ‘exotic’ and ‘velvet’ varieties. in the second experiment, the effect of various i...

present study was conducted to consider the effects of different concentrations of 2,4-d and ba on somatic embryogenesis induction, development and maturation of three strawberry cultivars (kurdistan, parose and camarosa). for this purpose, leaf blade, nodal, petiole, stamen and flower bud calli were cultured on murashige and skoog (ms) medium supplemented with 2, 4 dichlorophenoxyacetic acid (...

In vitro propagation of C. morifolium cv. ‘Homa’ and cv. ‘Delkash’ and wild C. coccineum via direct and indirect organogenesis and somatic embryogenesis were investigated. BAP at 0, 1, 2 and 3 mg l-1 or NAA at 0, 0.05, 0.1 and 0.2 mg l-1 concentrations were used to induce direct and indirect organogenesis of shoot tip explants. To study the callu...