bone tissue engineering has been one of the most promising areas of research, providing a potential clinical application to cure bone defects. recently, various stem cells, including embryonic stem cells (escs), bone marrow-derived mesenchymal stem cells (bm-mscs), umbilical cord blood-derived mesenchymal stem cells (ucb-mscs), adipose tissue-derived stem cells (adscs), muscle-derived stem cell...

background: embryonic stem cells (esc) are pluripotent cells which have been used as a model to study the mechanism that control the embryogenesis and early mammalian development in vitro. the aim of this study was to isolate and produce embryonic stem cells from late blastocyst stage embryos in mice. materials and methods: blastocyst stage embryos from pregnant nmri mice were obtained and cult...

introduction: the aim of this study was evaluate the ability of notochord to induce neural induction and/or differentiation of mouse embryonic stem cell to neuron and motor neuron, respectively. methods: in order to produce embryoid bodies, es cells line royan b1 were grown in suspension in the absence of lif for 4 days. ebs were divided into 4 groups. ebs in group 1 & 2 were further cultured i...

background: diabetes mellitus (dm), simply known as diabetes, refers to a group of metabolic diseases in which there are high blood sugar levels over a prolonged period. in this study, the feasibility and safety of intravenous transplantation of very small embryonic like stem cells (vsels) were investigated for diabetes repair, and finally the migration and distribution of these cells in hosts ...

The Rockefeller University Press $30.00 J. Cell Biol. Vol. 208 No. 4 443–455 www.jcb.org/cgi/doi/10.1083/jcb.201406102 JCB 443 Correspondence to Jason M. Haugh: [email protected] Abbreviations used in this paper: FMI, forward migration index; FP, fluorescent protein; GPI, glycophosphatidylinositol; MEF, mouse embryonic fibroblast; PDMS, polymethylsiloxane; PI3K, phosphoinositide 3-kinase; PI...

Nanoceria (cerium dioxide nanoparticles, CeO2) has a broad range of biological properties including antiviral activity. The hypothesis was that nanoceria can efficacy against coronavirus (coronavirus porcine transmissible gastroenteritis) and potentially target SARS-CoV-2. Transmissible gastroenteritis (TGEV) is the etiologic agent (PTG), highly contagious pig intestinal disease. aim study to d...

Survival of 16,838 potential embryos was determined by counting corpora lutea and fetuses at 50 d of gestation for 1,081 litters by 225 sires. These data, coded as 1 or 0 depending on whether an ovulation was represented by a fetus, were used to estimate direct and maternal additive genetic variances and their covariance for embryonic survival. Data were from first-parity gilts of a Large White...

introduction: doxorubicin is frequently used for treatment of several types of cancer. doxorubicin cardiac toxicity has limited the use of this drug. corticosteroids may prevent doxorubicin induced cardiotoxicity. therefore the aim of this study was to evaluate mouse embryonic stem cells derived cardiomyocytes as a model to evaluate the effect of doxorubicin and dexamethasone. methods: mouse em...

in the present study, 2, 4-dichlorophenoxyacetic acid (2, 4-d) was assessed individually for its effectiveness to induce somatic embryogenesis in tea (camellia sinenesis l.). embryonic axes and cotyledons explants were dissected from the seeds. explants were cultured on murashige and skoog (ms) medium containing 0, 1 and 5 mm 2, 4-d alone for embryonic axes and 0, 1 and 5 mm 2, 4-d along with 0...