Abstract Purpose of Review Untreated wastewater discharge can significantly and negatively impact the state environment. Rapid industrialization economic development have directly contributed to land water pollution resulting from application many chemicals such as organic dyes, pharmaceuticals, industrial reagents. The removal these before effluent is crucial for environmental protection. This...

The Cover Feature shows the carrier-free immobilization of self-assembled multi-enzyme complexes formed on basis polypeptide interactions and enzyme oligomerization. Such supermolecular water-insoluble feature a high reaction rate good recycling capability for biological biocatalytic conversion starch into inositol. proposed strategy complex sheds light improving catalytic efficiency in vitro s...

We explore the means by which immobilization of a substrate on a surface can increase the rate of a diffusion-controlled enzymatic reaction. A quasichemical approach is developed and compared with Brownian dynamics simulations. We use these methods to show that restricting only the orientation of the enzyme by long-range interactions with the surface is sufficient for enhancing catalysis.

The use of enzymes as catalysts requires recovery and reuse to make the process viable. Enzymatic immobilization changes enzyme stability, activity, specificity. It is very important explore new substrates for with appropriate composition structure improve efficiency immobilized enzymes. This work explores two different graphene oxide papers, one produced by oxidation route (GO) other electroch...

In this work, a simple method for alcohol synthesis with high enantiomeric purity was proposed. For this, colloidal gold and silver surface modifications with 3-mercaptopropanoic acid and cysteamine were used to generate carboxyl and amine functionalized gold and silver nanoparticles of 15 and 45 nm, respectively. Alcohol dehydrogenase from Thermoanaerobium brockii (TbADH) and its cofactor (NAD...

The combinatorial synthesis of 2-aminophenoxazin-3-one (APO) in a microfluidic device is reported. Individual microfluidic chips containing metallic zinc, silica-immobilized hydroxylaminobenzene mutase and silica-immobilized soybean peroxidase are connected in series to create a chemo-enzymatic system for synthesis. Zinc catalyzes the initial reduction of nitrobenzene to hydroxylaminobenzene wh...

Over the last decade, there has been a wealth of application for immobilized and stabilized enzymes including biocatalysis, biosensors, and biofuel cells. In most bioelectrochemical applications, enzymes or organelles are immobilized onto an electrode surface with the use of some type of polymer matrix. This polymer scaffold should keep the enzymes stable and allow for the facile diffusion of m...

BACKGROUND β-Galactosidase is a vital enzyme with diverse application in molecular biology and industries. It was covalently attached onto functionalized graphene nano-sheets for various analytical applications based on lactose reduction. METHODOLOGY/PRINCIPAL FINDINGS Response surface methodology based on Box-Behnken design of experiment was used for determination of optimal immobilization c...