We report the first evidence of an enzyme-catalyzed transetherification of model 17 alkoxysilanes. During an extensive enzymatic screening in the search for new biocatalysts 18 for silicon-oxygen bond formation we found that certain enzymes promoted the 19 transetherification of alkoxysilanes when tert-butanol or 1-octanol were used as the 20 reaction solvents. 21

Horseradish peroxidase was encapsulated in calcium alginate for the purpose of phenol removal. Considering enzyme encapsulation efficiency, retention activity and enzyme leakage of the capsules, the best gelation condition was found to be 1 % w/v of sodium alginate solution and 5.5 % w/v of calcium chloride hexahydrate. Upon immobilization, pH profile of enzyme activity changes as it shows ...

An artificial ‘minipathway’ starting with oxyfunctionalization of readily available limonene to carveol followed by oxidation to the corresponding α,β-unsaturated ketone via an alcohol dehydrogenase, an enoate reductase for the subsequent reduction of the double bond and concluded by a Baeyer-Villiger monooxygenase for the formation of the corresponding lactone is presented. This extension of t...

Galacto-oligosaccharides (GOS), increasingly popular prebiotics, are synthesized by enzymatic conversion of lactose. Among others, the total production costs significantly influenced costly enzyme. Therefore, it was investigated if reuse and full recovery enzyme is feasible, followed development a semi-continuous process in order to maintain consistent high GOS yield. As preliminary step, enzym...

In this research, an enzyme modified electrode has been produced during the electropolymerization of aniline through incorporation of sulfite oxidase into a conducting polymer. Then the bioelectrochemical response of resulted sulfite biosensor was investigated at different experimental conditions. Study of  the stability of the resulted sulfite biosensor revealed that formation of a passive...

horseradish peroxidase was encapsulated in calcium alginate for the purpose of phenol removal. considering enzyme encapsulation efficiency, retention activity and enzyme leakage of the capsules, the best gelation condition was found to be 1 % w/v of sodium alginate solution and 5.5 % w/v of calcium chloride hexahydrate. upon immobilization, ph profile of enzyme activity changes as it shows high...

Deprotonation of carbon and decarboxylation at enzyme active sites proceed through the same carbanion intermediates as for the uncatalyzed reactions in water. The mechanism for the enzymatic reactions can be studied at the same level of detail as for nonenzymatic reactions, using the mechanistic tools developed by physical organic chemists. Triosephosphate isomerase (TIM) catalyzed interconvers...