BACKGROUND: Magnetic nanoparticles have been significantly used for coupling with biomolecules, due to their unique properties. METHODS: Magnetic nanoparticles were synthesized by thermal co-precipitation of ferric and ferrous chloride using two different base solutions. Glucose oxidase was bound to the particles by direct attachment via carbodiimide activation or by thiophene acetylation of ma...

Urate oxidase is a peroxisomal enzyme with four equal subunits that convert uric acid to allantoin, more soluble metabolite for excretion. The usage of uricase as drug in medicine treat hyperuricemia. Many microorganisms have been used production such Streptomyces exfoliates, Pseudomonas aeruginosa, and Aspergillus flavus. In this study, soil samples were collected then cultured screening mediu...

Diabetes is one of the most severe health diseases in world. More than 150 million people worldwide suffer from diabetes caused by an abnormal glucose concentration their blood and tissues. The analysis levels body significant, primarily due to mellitus. Therefore, accurately detecting crucial for diagnosing, treating, managing diabetes. Diabetic patients can self-manage condition monitoring le...

Titanium dioxide (TiO2) and Fe3O4 magnetite particles were coated on spherical Kissirises; glucose oxidase (GOx) enzyme was immobilized on Kissiris/Fe3O4/TiO2 by physical adsorption. This catalyst was analyzed by a scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), and energy dispersive X-ray (EDX) measurements. The performance of the prepared biocatalyst in the...

A typical glucose sensor is a oxidase (GOx) enzyme-based due to its high sensitivity and selectivity. However, it has difficulty activating stabilizing the enzyme. To enhance stability of GOx-based sensors, chitosan added ZnS-doped Mn nanoparticles. Chitosan stabilizes particles can induce charge processes such as creation, transfer, separation at polymer interface nanoparticles' interface. Hen...

An optimal environment for glucose oxidase (GOx) in Nafion membranes is achieved using an advanced immobilization protocol based on a nonaqueous immobilization route. Exposure of glucose oxidase to water-organic mixtures with a high (85-95%) content of the organic solvent resulted in stabilization of the enzyme by a membrane-forming polyelectrolyte. Such an optimal environment leads to the high...

We discuss, from an industrial point of view, the scope and possibilities of recombinant DNA technology for "diagnostic enzyme" production and application. We describe the construction of enzyme-overproducing strains and show how to simplify downstream processing, increase product quality and process profitability, improve diagnostic enzyme properties, and adjust enzymes to harsh assay conditio...

Antersera prepared against both enantiozymes, D- and L-6-hydroxynicotine oxidase, formed precipitins in double diffusion tests with their respective antigens only. A mixture of the two antisera caused spur formation of the two precipitin lines obtained with the pure enzymes. Antiserum to L-apoprotein reacted with native L-enzyme and L-apoprotein but not with the D-sspecific enzyme. D-6-hydroxyn...