An enzyme immunoassay for gentamicin utilizing glucose-6-phosphate dehydrogenase was compared to the radioimmunoassay. The enzyme immunoassay for gentamicin was accurate, specific, and easily performed. It offers an ulternative method to assay aminoglycosides and could be used in institutions using enzyme immunoassay to assay other drugs.

Enzyme-linked immunosorbent assay (ELISA) has been served as both the workhorse and gold standard in immunoassay for its high specificity, sensitivity, accuracy, despite known shortcomings limitations....