ELISA: enzyme-linked immunosorbent assay IIF: indirect Immunofluorescence PNP: paraneoplastic pemphigus PV: pemphigus vulgaris INTRODUCTION Because of variability in clinical and histopathologic findings, the diagnosis of paraneoplastic pemphigus (PNP) frequently relies on the combination of direct immunofluorescence and indirect immunofluorescence (IIF). Here we present a unique case of PNP as...

Indirect plate enzyme-linked immunosorbent assay was standardized and evaluated for its effectivenessin immunodiagnosis of haemonchosis in experimental and clinical cases in sheep by using somatic wholeadult antigen of H. contortus. Plate ELISA was standardized using 5 μg/well antigen concentration with1:100 and 1:1000 of sera and conjugate dilution. Indirect plate ELISA was able to demonstrate...

A rapid enzyme-linked immunosorbent assay (ELISA) where component incubation periods were shortened to one hour, was compared with agar gel double diffusion (AGDD) and a standard ELISA procedure for detecting antibodies to Aspergillus fumigatus in 28 asthmatic patients with suspected allergic aspergillosis. Using two A fumigatus antigens the rapid ELISA compared well with AGDD and the standard ...

A new generation biochip is described as capable of supporting high-throughput (HT), multiplexed enzyme-linked immunosorbent assays (ELISAs). These biochips consist of an optically flat, glass plate containing 96 wells formed by an enclosing hydrophobic Teflon mask. The footprint dimensions of each well and the plate precisely match those of a standard microplate. Each well contains four identi...

Hakkari ili domates ve biber üretim alanlarında lekeli solgunluk virüsü (Tomato spotted wilt virus, TSWV) yonca mozaik (Alfalfa mosaic AMV)’nün belirlenmesi için yapılan sürveyler 2020 döneminde gerçekleştirilmiştir. Domates sararma, nekroz, şekil bozukluğu simptomu gösteren 184 bitkiden yaprak örneği toplanmıştır. Toplanan örneklerinde TSWV AMV’nin Double antibody sandwich enzyme linked immuno...

The enzyme, xanthine oxidase, has been implicated as a major source of destructive free radicals in ischemia-reperfusion injury associated with a range of pathological states [l]. Enzymic activity has been detected in many mammalian tissues [4] by using, variously, spectrophotometric [51, radiometric[6] and fluorimetric [71 assays. Apart from bovine milk fat globule membrane, which has long pro...

6 Department of Parasitology and Parasitic Diseases, Faculty of Veterinary Medicine, University of 7 Agricultural Sciences and Veterinary Medicine Cluj-Napoca, Cluj-Napoca, Romania; Veterinary 8 Mycology, Department of Infectious and Parasitic Diseases, Faculty of Veterinary Medicine, 9 University of Liège, Liège, Belgium; Dermatology and Venereology Department, Laboratory of 10 Mycology, Centr...