The human cytomegalovirus UL80 protease was expressed in Escherichia coli and purified by metal-chelate chromatography using a histidine tag engineered at the amino terminus. Cleavage of the 30-kDa protease at an internal site, VEA/A144, resulted in the recovery of 16- plus 14-kDa two-chain protease. The amino-terminal 16-kDa chain and the carboxyl-terminal 14-kDa chain remained associated as a...

Hemin/G-quadruplex (hGQ) DNAzymes are horseradish peroxidase-mimicking catalysts capable of the oxidation a variety substrates. We now implement aptamer-functionalized hGQ DNAzymes, also known as nucleoapzymes, to achieve increased bioconjugation N-methyl luminol tyrosine-containing residues and peptides. found that presence tyrosinamide-binding aptamer leads 12-fold increase in catalytic rate ...

Mucopolysaccharidosis type IIIB is a devastating neurological disease caused by lack of the lysosomal enzyme, α-N-acetylglucosaminidase (NAGLU), leading to toxic accumulation heparan sulfate. Herein we explored pharmacological chaperone approach enhance residual activity NAGLU in patient fibroblasts. Capitalizing on three-dimensional structures two modest homoiminosugar-based inhibitors complex...

INTRODUCTION Dengue fever is an acute viral disease, which usually presents as a mild febrile illness. Patients with severe disease present with dengue haemorrhagic fever or dengue toxic shock syndrome. Rarely, it presents with abdominal symptoms mimicking acute appendicitis. We present a case of a male patient presenting with right iliac fossa pain and suspected acute appendicitis that was lat...

discussion hydatid cyst in pericardium represents only 0.5-2% of cases of systemic echinoccocal infection. isolated pericardial cyst is very rare in endemic region and may present mimicking acute coronary syndrome. cardiac hydatid cysts should always be considered in presence of eosinophilia as present like acute coronary syndrome in endemic area. introduction hydatid cysts are most commonly fo...

Nanofibers are considered versatile materials with remarkable potential in tissue engineering and regeneration. In addition to their extracellular matrix-mimicking properties, nanofibers can be functionalized specific moieties (e.g., antimicrobial nanoparticles, ceramics, bioactive proteins, etc.) improve overall performance. A novel approach this regard is the use of enzymes immobilized onto i...

Although nanozymes have been widely developed, directly utilizing light to drive catalytic reactions like natural photoenzymes still remains challenging. Herein, we propose that photonanozymes (PNZs), as a novel kind of nanozyme, exclusively possess enzyme-mimicking activity under illumination. Only in the presence visible light, as-synthesized TiO2 proposed this contribution shows excellent sp...

Urease, a nickel-containing metalloenzyme is getting remarkable attention due to diverse range of applications for mankind. Urease plays magnificent role in various field like agriculture, analytical, geological phenomena, beverage industry and an important diagnostic tool. mainly present bacteria, fungi, plants invertebrates its manifestation specific genera may open new vistas taxonomic posit...

The CD44+ and CD44- subpopulations of the colorectal cancer cell line Caco2 were analyzed separately for their sensitivities to the antitumor drug camptothecin. CD44+ cells were less sensitive to camptothecin than CD44- cells. The relative resistance of CD44+ cells was correlated with (i) reduced activity of the nuclear enzyme topoisomerase I and (ii) insensitivity of this enzyme to camptotheci...