A decision-making protocol/tool is developed in which bark analytical data can be the input to predict most appropriate enzymic systems employ.

The rapid loss of ATPase activity of myosin from the red, or slow, skeletal muscles of the rabbit under mild alkaline conditions has been studied in detail. The loss of ATPase activity is irreversible and consistent with a mechanism involving two !irst order reactions with rate constants of 0.3 and 0.007 min-l. No gross change in molecular structure accompanies inactivation, as judged by the la...

Webster (1) first noticed that 2,4-dinitrophenol increased the ATPase” activity of myosin. Attention was focused on this observation by the work of Greville and Needham (2) and Chappell and Perry (3), who showed that under certain conditions DNP accelerated the ATPase activity of myosin A, myosin B, and myofibrils, whereas the ITPase activity was inhibited. They noted the similarity between the...

Poly(ADP-ribose) polymerase-1 (PARP-1) is a co-activator for AP-2alpha (activator protein 2alpha)-mediated transcriptional activation. In the present study, we find that the role of PARP-1 in AP-2alpha transcription is distinctly dualistic with opposing effects. Separate regions of PARP-1 interact with AP-2alpha and independently control its transcriptional activation. The C-terminus containing...

Solubilization and partial purification of the microsomal UDP-glucose sterol glucosyl transferase activity from maize coleoptiles by chromatography on DEAE-cellulose resulted in a highly delipidated (>95%) and inactive enzymic preparation. Addition of sterols revealed part of the activity and subsequent addition of phospholipids further increased the activity. Negatively charged phospholipids w...

An enzyme which catalyzes the formation of nicotinamide mononucleotide in the presence of nicotinamide, S-phosphoribosylpyrophosphate, adenosine triphosphate, and Mgff has been purified from rat liver. The reaction is highly specific for ATP and is not substituted for by a variety of nucleotides. Enzymic activity has been found in all of the tissues studied, and in the crude homogenate it is ap...

Incubation of pig kidney microvillar membranes with Bacillus thuringiensis or Staphylococcus aureus phosphatidylinositol-specific phospholipase C (PI-PLC) resulted in the release of a number of glycosyl-phosphatidylinositol (GPI)-anchored hydrolases, including alkaline phosphatase (EC 3.1.3.1), amino-peptidase P (EC 3.4.11.9), membrane dipeptidase (EC 3.4.13.19), 5'-nucleotidase (EC 3.1.3.5) an...

1. Seven unique carboxymethylcysteine-containing peptides have been isolated from tryptic digests of rabbit muscle aldolase carboxymethylated with iodo[2-(14)C]acetic acid in 8m-urea. These peptides have been characterized by amino acid and end-group analysis and their location within the cyanogen bromide cleavage fragments of the enzyme has been determined. 2. Reaction of native aldolase with ...