a random 12 mers phage library was used to screen a pool of immunoglo¬bulin fractions obtained from vitiligo patients. subsequent to panning experiments, a panel of affinity selected phage from vitiligo patients were obtained. this panel was tested using an elis a for their reactivity with pooled sera from patients and normal controls. among the 16 randomly selected clones, two of clones showed...

With the aim of the production of human factor VIII antigen and its corresponding antibody an epitope coding fragment of the light-chain of hFVIII, fused to a His6-tag, was isolated and over-expressed in Escherichia coli. The over-expressed hFVIII-epitope containing peptide was confirmed by its reaction with a rabbit serum directed against native hFVIII as well as antiHis6-tag antibody. An expr...

Pemahaman konsep merupakan kemampuan penting yang dimiliki siswa dalam mempelajarai IPA di sekolah dasar. Siswa memahami dapat mengetahui hubungan satu sama lain sehingga bisa diterapkan untuk memecahkan masalah. Namun dari hasil observasi diketahui pemahaman kelas V SD 03 Puri Pati masih rendah. Penelitian ini bertujuan meningkatkan dengan model pmebelajaran mind mapping berbantuan media manip...

Abstract B-cells inducing antigen-specific immune responses in vivo produce large amounts of antibodies by recognizing the subregions (epitope regions) antigen proteins. They can inhibit their functioning binding to Predicting epitope regions is beneficial for design and development vaccines aimed induce antibody production. However, prediction accuracy requires improvement. The conventional re...

Genetic analysis of HIV-1 is essential to improve treatment strategies and select epitopes for vaccine programs. The objective of this study was to determine whether known CD4+ and CD8+ epitopes were present in Brazilian HIV-1 strains. We used previously described CD8+ and CD4+ epitopes from the Los Alamos laboratory to search for these epitopes in the Brazilian sequences using the HIVbase prog...

Epitope mapping from affinity-selected peptides has become popular in epitope prediction, and correspondingly many Web-based tools have been developed in recent years. However, the performance of these tools varies in different circumstances. To address this problem, we employed an ensemble approach to incorporate two popular Web tools, MimoPro and Pep-3D-Search, together for taking advantages ...

Infectious bursal disease (IBD), a highly contagious and devastating disease in young chicken, is causedby infectious bursal disease virus (IBDV). To improve the immunogenicity of recombinant IBDV subunitvaccine, an attempt was made to find a new way to prepare IBD vaccine containing glycosylated mVP2antigen. Firstly, IBDV mVP2 gene (with a nucleic acid sequence encoding B cell epitope of IBDV(...