نتایج جستجو برای: equine mscs isolation
تعداد نتایج: 151653 فیلتر نتایج به سال:
The use of mesenchymal stem cells (MSCs) in human and veterinary clinical applications has become a subject increasing importance due to their roles immunomodulation regenerative processes. MSCs are especially relevant equine medicine because they may have the ability treat prevalent musculoskeletal disorders, among other conditions. However, recent evidence suggests that components secreted by...
Umbilical cord derived mesenchymal stromal cells (UC-MSCs) are a focus for clinical translation but standardized methods for isolation and expansion are lacking. Previously we published isolation and expansion methods for UC-MSCs which presented challenges when considering good manufacturing practices (GMP) for clinical translation. Here, a new and more standardized method for isolation and exp...
Background: Tendon injuries are a major cause of orthopaedic injuries, and often compromise the return to the same performance level. Therefore, different regenerative therapies, such as Mesenchymal Stem Cells (MSCs) and Platelet-rich Plasma (PRP) have been explored to improve tendon healing in horses. However, ectopic bone formation of undifferentiated cells is a major concern, because of repo...
Objective- To evaluate growth characteristics and differentiation capacity of equine mesenchymal stem cell (eMSCs) derived from bone marrow (BM). Study design- In vitro experimental study. Animals- Four young adult horses (2-5 years old) Procedure- Cell morphology and growth characteristics of eMSCs harvested from BM were evaluated in standard culture conditions. eMSCs in passage 3 were subj...
A cross-sectional study was carried out from November 2016 to May 2017 isolate and identify Clostridium tetani. total of 71 samples (equine deep wound swabs, feces, soil the feces contaminated environment) were collected. Isolation tetani using an anaerobic Viande et Foie (VF) medium. Out cultured on VF medium, 27 (38%) them grown all confirmed be spore staining biochemical tests. Study site sa...
BACKGROUND Mesenchymal stromal cells (MSCs) are multipotent progenitor cells that originally derived from bone marrow. Clinical use of bone marrow-derived MSC is difficult due to morbidity and low MSC abundance and isolation efficiency. Recently, MSCs have been isolated from various adult tissues. Here we report the isolation of adenoid tissue-derived MSCs (A-MSCs) and their characteristics. ...
We describe the isolation of a paramyxovirus from three species of flying fox in Queensland. The species are Pteropus alecto (black flying fox), Pteropus scapulatus (little red flying fox) and Pteropus poliocephalus (grey headed flying fox). The virus appears to be identical to the equine morbillivirus which was associated with acute equine respiratory syndrome and two human deaths in two Queen...
background isolation of adipose tissue mesenchymal stem cells (mscs) with current enzymatic methods has some limitations. for example, it is costly and time-consuming and results in a heterogeneous cell population that making compromise proliferation and differentiation of mscs. also, it is accompanied with the increased risk of culture contaminations because of several handling steps.in this ...
Since the discovery of bone marrow derived stromal cell osteogenesis in the 1960s, tissue engineering with adult multipotent stromal cells (MSCs) has evolved as a promising approach to restore structure and function of bone compromised by injury or disease. To date, accelerated bone formation with MSCs has been demonstrated with a variety of tissue engineering strategies. Though MSC bone tissue...
Tendons transmit forces generated by muscle to move the joints they cross. Tendon problems are complicated by slow and incomplete healing as well as re-injury. Mesenchymal stem cell based therapies show promise in improving outcomes. Much of the work has been experimental, although early clinical use in equine strain-induced tendon injury supports the efficacy of this strategy. While much has b...
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