Erythromycin resistance among streptococci is commonly due to target site modification by an rRNA-methylating enzyme, which results in coresistance to macrolide, lincosamide, and streptogramin B antibiotics (MLSB resistance). Genes belonging to the ermAM (ermB) gene class are the only erythromycin resistance methylase (erm) genes in Streptococcus pyogenes with MLSB resistance that have been seq...

Faecal samples from 50 pigeons all originating from different lofts were screened for the presence of macrolide and lincosamide (ML)-resistant isolates of Streptococcus gallolyticus and Enterococcus columbae by plating the samples onto selective media. Sixty-eight ML-resistant E. columbae strains were recovered from the faecal samples of 29 animals. Two of these samples also harboured ML-resist...

The macrolides appear to have considerable effects for treatment of bovine mastitis because of excellent diffusion into the mammary gland, long half-life, low protein binding, high intracellular concentration and lipid solubility. Acquired resistance to macrolides in Staphylococcus aureus is primarily related to target-site modification through acquisition of an erm gene. In t...

One hundred and twenty-four erythromycin-resistant pneumococcal isolates were examined for the presence of macrolide resistance genes. The erm(B) gene was detected in 118 (95.2%) isolates and the mef(A) gene in 83 (66.9%) isolates. Both the mef(A) and erm(B) genes were detected in 77 (62.1%) isolates. DNA macrorestriction analysis of these isolates identified them as belonging to a single multi...

PIWI-interacting RNAs (piRNAs) are thought to silence transposon and gene expression during development. However, the roles of piRNAs in somatic tissues are largely unknown. Here we report the identification of 555 piRNAs in human lung bronchial epithelial (HBE) and non-small cell lung cancer (NSCLC) cell lines, including 295 that do not exist in databases termed as piRNA-like sncRNAs or piRNA-...

disc testing was used to determine antibiotic susceptibility and erythromycin resistance phenotypes. DNA from test samples was prepared by both Qiagen extraction and rapid boiling. As the efficiencies of the two methods were comparable, the rapid boiling method was used throughout. Primers were as published for erm(A), erm(B), erm(C), mef(A/E) 5 and erm(TR). 4 Donor and acceptor probe-pairs wer...

background and objectives: macrolide, lincosamide and streptogramin type b (mlsb) antibiotics are important in the treatment of staphylococcus aureus infections and existence of isolates with ability to resist against mlsb antibiotics is worrisome. materials and methods: in this cross sectional study, 101 s. aureus isolates were collected from patients of five selected hospitals in tehran over ...

OBJECTIVES To determine macrolide-lincosamide-streptogramin (MLS) resistance determinants in the Capnocytophaga genus and to describe the prevalence of β-lactam resistance genes in human oral Capnocytophaga species. METHODS Forty-eight Capnocytophaga isolates identified by analysis of 16S rRNA sequences were isolated from subgingival samples from 14 haematology patients (HPs), 11 periodontiti...

BACKGROUND We identified erm(A)-harbouring Streptococcus pyogenes that expressed three variant phenotypes: (1) low-level resistance to erythromycin (MICs 1-4 mg/L) but high azithromycin MICs in absolute terms (16-64 mg/L; n=6); (2) same as (1) but with a high clindamycin MIC (256 mg/L; n=1); and (3) high-level constitutive MLS (cMLS) resistance (n=1). Here we analysed the genetic basis of these...