conclusions: e. coli herb-resistance plasmid can replicate and be expressed in s. aureus. results: the identified 45 kb herb-resistance plasmid could be transferred from e. coli cp9 isolates to e. coli dh5α. as a consequence e. coli dh5α transconjugant mic increased from 0.0125 g/ml to 0.25 g/ml. the plasmid was easily transferred from e. coli cp9 strain to s. aureus rn450rf with a mean transfe...

conclusions reteplase was expressed in e. coli top 10 after activation of pbad/giiia promoter region by arabinose and optimized. results the obtained recombinant plasmid was sequenced to confirm the presence and correct framing of reteplase gene regarding the expression of reteplase. maximum production of this enzyme was obtained under the following condition: 0.0002% l-arabinose at 37°c for 2 ...

this study was carried out to determine the prevalence of foodborne pathogens, escherichia coli, e. coli o157:h7, listeria monocytogenes and campylobacter spp. on slaughtered cattle in isfahan, iran. a total of 203 cattle carcasses were sampled by surface section of neck meat taken immediately after slaughter and analyzed using microbiological examinations. suspected colonies to e. coli o157:h7...

background: there is a growing concern on the impact of the presence of extended-spectrum β-lactamase (esbl) producing escherichia coli isolated from animals on public health. objectives: the aim of this study was to investigate the presence of three classes of esbl genes in e. coli isolates from sheep and broilers at a slaughter in urmia region, iran. methods: a total of 111 e. coli isolates w...

Chenopodium album is a weedy annual plant in the genus Chenopodium. C. album pollen represents a predominant allergen source in Iran. The main C. album pollen allergens have been described as Che a 1, Che a 2, and Che a 3. The aim of this work was to clone the Che a 1 in Escherichia coli to establish a system for overproduction of the recombinant Che a 1 (rChe a 1). In order to clone this aller...

conclusions our results showed that the recombinant protein produced by the pbad vector in the escherichia coli system was very efficient. background nontypeable haemophilus influenzae (nthi) is a significant pathogen in children, causing otitis media, sinusitis, conjunctivitis, pneumonia, and occasionally invasive infections. protein d (pd) belongs to the minor outer-membrane proteins of h. in...

results the results showed that the chimeric recombinant protein induced strong humoral response as well as protection against live oral challenges using e. coli o157:h7. conclusions the rit could reduce bacterial shedding effectively. objectives the eae gene encoded intimin protein which is essential for colonization of the mucosa and a/e lesions formation by docking to the tir. materials and ...

The effects of five tetracycline analogues, anhydrotetracycline, doxycycline, minocycline, oxytetracycline, and tetracycline, on Top10 promoter activity in NT1 tobacco tissue culture cells have been analysed. The concentration that repressed Top10 promoter activity, the level of transgene repression and the kinetics of transgene de-repression were determined for each analogue, and could not be ...

conclusions ctx-m was the most prevalent esbl genotype in uropathogenic e. coli (upec) isolated from uti. in addition, a high frequency of qnr genes among esbl-producing e. coli was identified in this study. in order to avoid treatment failures, we recommend using phenotypic and molecular methods to diagnose these enzymes and qnr genes. results the highest sensitivity was seen to imipenem (96.7...

conclusions the impact of sugars and other factors on biofilm formation can vary depending on the type of bacteria present. results results showed that the presence of sugars has no effect on the bacterial growth rate; all three concentrations of sugars were hydrophilic and demonstrated a high affinity toward binding to the surfaces. background bacteria utilize various methods in order to live ...