The presence of microbial load and high fiber content in various non conventional feedstuffs limit their utilization in poultry feed. In the present study, the feedstuffs were treated with acidified sodium chlorite (ASC) and its impact on  the microbial load and metabolizable energy availability was assessed in  the chickens. The effect of supplementation of feed grade enzyme was also evaluated...

Mitochondrial 26-hydroxylation of exogenous cholesterol, endogenous cholesterol, and 5beta-cholestane-3alpha,7alpha,12alpha-triol was studied. 26-Hydroxylation of endogenous cholesterol was measured by mass fragmentography. NADPH and isocitrate stimulated 26-hydroxylation of endogenous as well as exogenous cholesterol. 26-Hydroxylation of endogenous cholesterol was linear with time for 15 min, ...

An exogenous redox-free, membrane-less enzyme biofuel cell-based ultrasensitive self-powered cytosensing platform was fabricated. With the ultrahigh sensitivity and the merits of not requiring external power sources or exogenous reagents, the device has great potential as a point-of-care tool for early diagnosis of cancer in vivo.

A 2 × 3 factorial experiment was conducted to investigate the effect of enzyme Endofeed W (EEW) at 0 and 0.05%, thyme essential oil (TEO), probiotic Primalac® (PP) and non-feed additive, on intestinal histomorphology of broilers fed wheat-based diet. Each of the 6 dietary treatments was replicated 4 times with 12 chicks each from 0 to 42 days of age. There was no interaction between enzyme and ...

Acyl-coenzyme A:cholesterol acyltransferase (ACAT) is the intracellular enzyme responsible for the esterification of cholesterol with long-chain fatty acyl-CoA derivatives and has been implicated in atherosclerosis and gallstone disease. The effects of exogenous cholesterol and dithiothreitol (DTT) on the ACAT activity of human liver microsomes have been determined. Pre-incubation of microsomes...

BACKGROUND Amidation of the carboxyl terminal of many peptides is essential for full biological potency, often increasing receptor binding and stability. The single enzyme responsible for this reaction is peptidylglycine α-amidating monooxygenase (PAM: EC 1.14.17.3), a copper- and ascorbate-dependent Type I membrane protein. METHODS To make large amounts of high molecular weight amidated prod...

Objective: To explore the protective role of exogenous Creatine Phosphate (PCr) in the postoperative cardiac muscle recovery in children undergoing open heart surgery. Material and methods: 63 cases of congenital heart disease were randomly divided into 3 groups; A, B and C; 21 cases in each group. Myocardial perfusion fluids were made of the same amount of blood in the St, Thomas Hospital card...

The effects of pre-treating different types of roughages with alkali on the efficacy of exogenous fibrolytic enzymes for improving their digestibility were studied in vitroin factorial arrangement 4 × 3 × 5 (enzyme, treatment and roughage types). Two fibrolytic feed enzymes novozyme (N) and celloclast (C) their combination (N+C) were evaluated for their potential to improve in vitrodegradation ...