نتایج جستجو برای: explants

تعداد نتایج: 7790  

Journal: :Plant physiology 1966
F B Abeles R E Holm

Ethylene stimulated RNA and protein synthesis in bean (Phaseolus vulgaris L. var. Red Kidney) abscission zone explants prior to abscission. The effect of ethylene on RNA synthesis and abscission was blocked by actinomycin D. Carbon dioxide, which inhibits the effect of ethylene on abscission, also inhibited the influence of ethylene on protein synthesis. An aging period appears to be essential ...

Journal: :Plant physiology and biochemistry : PPB 2005
Luis Arigita Belén Fernández Aida González Ricardo Sánchez Tamés

In traditional in vitro culture, explants grow enclosed in a non-ventilated vessel at high relative humidity with phytohormones continuously present and sucrose as the main energy source. Under such conditions explant growth is far from normal. In this paper, explants of Actinidia deliciosa were cultured in MS medium supplemented with sucrose, benzyladenine and gibberellic acid under autotrophi...

Journal: :Investigative ophthalmology & visual science 2003
Cecilia V Rojas Jessica I Martínez Ingrid Flores Dennis R Hoffman Ricardo Uauy

PURPOSE To explore the effect of docosahexaenoic acid (DHA) on gene expression during human fetal retinal maturation. METHODS Human fetal retinal explants were cultured in serum-free Waymouth's medium supplemented with DHA or oleic acid (OA), using bovine serum albumin (BSA) as the vehicle. After 14 days in culture, fatty acid composition was assessed, and the abundance of 2400 cDNAs was exam...

Journal: :Annals of the rheumatic diseases 1996
X Chevalier P Claudepierre N Groult G J Godeau

OBJECTIVE To determine the influence of IL-1 beta on the presence and the distribution of tenascin in matrix of human normal and osteoarthritic cartilage explants. METHODS Cartilage was grown in organotypic culture with or without IL-1 beta (10 ng ml1). Tenascin antigen was detected on cryopreserved cartilage sections by immunohistochemical techniques with a monoclonal antibody directed again...

Journal: :Horticulturae 2021

Peppers (Capsicum spp.) are recalcitrant to in vitro culture regeneration, making the application of vitro-based breeding strategies difficult. We evaluated impact different combinations auxins, cytokinins and micronutrients on induction direct organogenesis cotyledon hypocotyl explants C. annuum, baccatum chinense. found variation regeneration response among species type explant. In this way, ...

2013
Abigail L Clutterbuck David Allaway Pat Harris Ali Mobasheri Stefan Toegel Ali Mobasheri Oliver Grundmann

OBJECTIVE Curcumin (diferuloylmethane) is a phytochemical with potent anti-inflammatory and anti-oxidant properties, and has therapeutic potential for the treatment of a range of inflammatory diseases, including osteoarthritis (OA). The aim of this study was to determine whether non-toxic concentrations of curcumin can reduce interleukin-1beta (IL-1β)-stimulated inflammation and catabolism in a...

2013
Pawel Tulinski Ad C. Fluit Jos P. M. van Putten Alain de Bruin Sarah Glorieux Jaap A. Wagenaar Birgitta Duim

Staphylococcus aureus is an opportunistic pathogen able to colonize the upper respiratory tract and skin surfaces in mammals. Methicillin-resistant S. aureus ST398 is prevalent in pigs in Europe and North America. However, the mechanism of successful pig colonization by MRSA ST398 is poorly understood. To study MRSA colonization in pigs, an ex vivo model consisting of porcine nasal mucosa expla...

2016
D. K. Das A. Rahman

In the research it was examined the changes in total phenolic contents and seven major phenolic compounds (gallic acids, +(−) catechin, catechol, Chlorogenic acid, o-coumaric acid, rutin and quercetin) of two litchi cultivars (Purbi and Bedana) shoot tips and fruits (for zygotic embryos) collected in different months, in order to determine their effects on the explants browning during establish...

Journal: :Journal of cell science 1982
G W Ireland C D Stern

Cell-substrate contacts in explants of different regions of early chick tissues were investigated using the technique of interference reflection microscopy. All the explants spread as epithelial sheets. During initial spreading a peripheral zone of 2-3 cells formed broad contacts with the substrate. In spread explants some cells in the centre made broad substrate contacts. A mat of extracellula...

2015
Cara I. Robison Michael W. Orth

Objective: To characterize the effects of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) and arachidonic acid (ARA) in various combinations on indices of inflammation in recombinant porcine IL-1 beta stimulated porcine articular cartilage explants. Methods: Cartilage was obtained from the humeral-ulnar joints of Yorkshire x Landrace market sized gilts. Explants were harvested from t...

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