نتایج جستجو برای: expression analysis
تعداد نتایج: 3512037 فیلتر نتایج به سال:
background and aims: the aim of this study was cloning and expression of rabies virus glycoprotein by a eukaryotic expression plasmid pcdna3.1(+) in bsr cell line. this construct might be used for a potential dna vaccine. materials and methods: glycoprotein gene was synthesized and cloned into pbluescript vector and then sub cloned into eukaryotic expression vector (pcdna3.1(+)). after verifica...
abstract this study evaluated and compared medical terminology and english for the students of medicine (ii) as two representatives of the textbooks available on the iranian market for teaching english to the students of medicine. this research was performed on the basis of a teacher’s and a number of students’ attitudes and the students’ needs analysis for two reasons: first, to investigate...
Purpose: Effect of lipocalin 2 on the expression of heme oxygenase I , II and NF-kB transcription factor was the purpose of this survey. Materials and Methods: Lcn2 was cloned to pcDNA3.1 plasmid by using genetic engineering method. The recombinant vector was transfected to CHO and HEK293T to establish stable cell expressing lipocalin 2. The presence of lipocalin 2 gene in these cells was confi...
Background: Baculovirus expression system is one of the most attractive and powerful eukaryotic expression systems for the production of recombinant proteins. The presence of a biomarker is required to monitor transfection efficiency or protein expression levels in insect cells. Methods: The aim of this study was to construct a baculovirus expression vector encoding a copepod super green fluore...
congenital factor xiii deficiency is a very rare bleeding disorder, but because of the high rate of consanguineous marriages, it is common in sistan and baluchestan province of iran. the discovery of promoter hypermethylation of numerous mirnas in human diseases has demonstrated an epigenetic mechanism for aberrant mirna expression. the present study has analyzed methylation and expression stat...
Background: Maternal mRNA degradation is a selective process that occurs in waves corresponding to important developmental transitions such as resumption of meiosis, fertilization and zygotic genome activation. It has been demonstrated that the number, position, and combination of 3 UTR cis-acting elements interacting with trans-acting protein factors regulate translation and mRNA stability. Ou...
Salinity is one of the environmental stresses that affects bread wheat grain yield in most parts of the world. One of the basic strategies to mitgiate the effect of non-biological stresses such as salinity is genetic improvement of crop plants. Identification of stress-associated genes is a prerequisite for genetic improvement. In the present study, the role of a number of genes in the aquapori...
results following ectopic expression of mir-375, sox2ot and sox2 expression analysis revealed a significant decrease in their expression level (p < 0.05) in nt-2 cells compared to the control. cell cycle analysis following ectopic expression of mir-375 in the nt-2 cells using propidium iodine staining revealed significant extension in sub-g1 cell cycle. conclusions this is the first report to s...
مقایسه دو زبان فارسی و فرانسه در سطح آوایی موضوع پژوهش حاضر است . این مقایسه نشان داده است که زبان فرانسه دارای 18 همخوان و 10 واکه دهانی و 3 واکه غنه است . واکه های دهانی با مشخصات پیشین ˆ پسین و بازی ˆ بستگی و واکه های غنه با مشخصات غنه، لبی و پیشین ˆ پسین طبقه بندی شده اند. در زبان فارسی و فرانسه دیفتانگ وجود ندارد، د حالیکه در فرانسه قدیم نه تنها واکه های دوتایی بلکه واکه های سه تایی نیز وج...
In this study, our previously reported novel synthetic gene encoding 166 residues of interferon-? was used for an efficient expression of IFN-?. The synthetic gene was cloned into pET21a expression vector and transferred into E. coli. Recombinant protein was over-expressed in the E. coli. Identity of the recombinant protein was confirmed by western blot analysis. The recombinant protein was bio...
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