Non-nucleoside reverse-transcriptase inhibitors (NNRTIs) are one of the key components of antiretroviral drug regimens against human immunodeficiency virus type 1 (HIV-1) replication. (+)-calanolide A, which is purified from the rainforest plant Calophyllum, is an anti-HIV-1/ tuberculosis drug. In our previous study, a library of (+)-calanolide A was constructed and one novel compound 10-chloro...

Sato J.P.H., Takeuti K.L., Andrade M.R., Koerich P.K.V., Tagliari V., Bernardi M.L., Cardoso M.R.I. & Barcellos D.E.S.N. 2016. Virulence profiles of enterotoxigenic Escherichia coli isolated from piglets with post-weaning diarrhea and classification according to fecal consistency. Pesquisa Veterinária Brasileira 36(4):253-257. Setor de Suínos, Faculdade de Veterinária, Universidade Federal do R...

Adherence of F18 fimbrial Escherichia coli to porcine intestinal epithelial cells is mediated by the adhesin (FedF) of F18 fimbriae. In a previous study, we demonstrated the specificity of the amino acid residues between 60 and 109 as the receptor binding domain of FedF. In this study, different expression, secretion, and anchoring systems for the receptor binding domain of the FedF adhesin in ...

Figure 1: Illustration of stimuli used during test trials. Functions are reconstructed from human clicked locations. F18 is the Dirac function with a non-zero element at-270.

Enzyme-based metabolic mechanisms of insecticide resistance were investigated, comparing a deltamethrin-susceptible parent stock and resistant colonies of Anopheles minimus species A using biochemical assays. The control parent colony was determined susceptible to the diagnostic lethal concentration of deltamethrin (0.05%), whereas the 6 resistant test populations at selected 4, 8, 12, 14, 16, ...

BACKGROUND Post-weaning diarrhoea (PWD), due to Escherichia coli, is an important cause of economic losses to the pig industry primarily as a result of mortality and worsened productive performance. In spite of its relevance, recent data about the prevalence of virulence genes and pathotypes among E. coli isolates recovered from cases of PWD in Europe are scarce. RESULTS This study investigat...

HIV-1 entry into cells is mediated by interactions between the envelope (Env) gp120 and gp41 proteins with CD4 and chemokine receptors via an intermediate called the viral fusion complex (vFC). Here, mAbs were used to find the dynamic changes in expression of antigenic epitopes during vFC formation. A CD4-specific mAb (R275) and anti-vFC mAbs, designated F12-1, F13-6 and F18-4 that recognize th...

Identification of Escherichia coli causing porcine postweaning diarrhea requires knowledge regarding the prevalent pathotypes within a given region. A total of 100 Escherichia coli isolates from piglets with diarrhea in Londrina city, Parana State, South Brazil, were screened for the presence of genes for F4, F5, F6, F18, F41 fimbrial antigens by specific probes and for enterotoxins (STa, STb, ...

A polyphasic study was undertaken to establish the taxonomic position of a rhizosphere isolate that had been assigned provisionally to the genus Kitasatospora. The organism, isolate F18-98(T), was found to have chemical and morphological properties that were consistent with its classification as a Kitasatospora strain. Direct 16S rDNA sequence data confirmed the taxonomic position of the strain...

Converging evidence implicates the dopaminergic system and the prefrontal and nigrostriatal regions in the pathophysiology of attention deficit hyperactivity disorder (ADHD). Using positron emission tomography (PET) with [fluorine-18]fluorodopa (F18-DOPA), we compared the integrity of the presynaptic dopaminergic function between 17 ADHD adults and 23 healthy controls. The ratio of the isotope ...