Abstract Fatty acid-binding protein 5 (FABP5) is highly expressed in hepatocellular carcinoma (HCC) tissues and related to HCC progression. In this study, we analyzed the potential of serum FABP5 (sFABP5) as a tumor marker its clinical significance We compared sFABP5 concentration patients with (HCC group) that hepatitis without (hepatitis group). Moreover, measured expression levels resected (...

BACKGROUND Sebaceous cell carcinoma of the eyelid is a malignant tumor. However, the pathoetiology of sebaceous cell carcinoma is not clear. Retinoic acid (RA) signaling is essential for skin epidermal differentiation including the eyelids. In this study, we investigate the expression of β-catenin, RA-binding proteins and RA receptors in sebaceous cell carcinoma of the eyelid and try to estimat...

The role of PPARβ/δ in breast carcinogenesis remains unclear because some studies show that activating this receptor promotes breast cancer cell growth, while other studies show that activating this receptor inhibits breast cancer cell growth. Further, some studies suggest that the function of PPARβ/δ can be altered by the presence of fatty acid binding protein 5 (FABP5) and cell retinoic acid ...

BACKGROUND Human head and neck squamous cell carcinoma (HNSCC) is an aggressive and recurrent malignancy. Identification of unique or overexpressed cell-associated or cell surface antigens is critical for diagnosis and development of cancer vaccines and targeted therapies for HNSCC. We have used high throughput microarray technology to search for candidate targets in HNSCC. METHODS Gene expre...

Previous studies with mice lacking epidermal macroautophagy suggest that under pro-aging stress there is a profound change in lipid metabolism and an increased susceptibility to DNA damage cellular senescence. Thus we followed up these now cohorts of young (< 8 mo) geriatric (>16 which did or not lack Atg7 (Atg7 f/f: K14 cre+), studied the consequences chronological aging on level lipidome. Add...

Extracellular vesicles (EVs) are microvesicles secreted from various cell types. We aimed to discover a new biomarker for high Gleason score (GS) prostate cancer (PCa) in urinary EVs via quantitative proteomics. EVs were isolated from urine after massage from 18 men (negative biopsy [n = 6], GS 6 PCa [n = 6], or GS 8-9 PCa [n = 6]). EV proteins were labeled with iTRAQ and analyzed by LC-MS/MS. ...

Nuclear receptor-mediated signaling via RARs and PPARδ is involved in the regulation of skin homeostasis. Moreover, activation of both RAR and PPARδ was shown to alter skin inflammation. Endogenous all-trans retinoic acid (ATRA) can activate both receptors depending on specific transport proteins: Fabp5 initiates PPARδ signaling whereas Crabp2 promotes RAR signaling. Repetitive topical applicat...

Securinine, a GABA(A) receptor antagonist, has been reported to enhance monocyte cell killing of Coxiella burnetii without obvious adverse effects in vivo. We employed multiplex 2D gel electrophoresis using Zdyes, a new generation of covalently linked fluorescent differential protein detection dyes to analyze changes in the monocyte proteome in response to Securinine. Securinine antagonism of G...

The endocannabinoid anandamide (AEA) is an antinociceptive lipid that is inactivated through cellular uptake and subsequent catabolism by fatty acid amide hydrolase (FAAH). Fatty acid binding proteins (FABPs) are intracellular carriers that deliver AEA and related N-acylethanolamines (NAEs) to FAAH for hydrolysis. The mammalian brain expresses three FABP subtypes: FABP3, FABP5, and FABP7. Recen...