Excretory-secretory products (ESP) play an important role in the host biochemical defense by means of activities of detoxifying and antioxidant enzymes. The aim of this study was to evaluate praziquantel (PZQ) effects by detection of glutathione S- transferase (GST) and superoxide dismutase (SOD) enzymes activities in ESP samples of Fasciola spp. Fasciola gigantica and Fasciola hepatica a...

background: the trematodes of the genus fasciola (the liver flukes) are among the well-known instances of food-borne parasites worldwide. differentiation of fasciola species is important because of their different transmission and epidemio-logical characteristics. the current study was undertaken to discriminate fasciola species in the domestic ruminants of urmia city, iran. methods: adult fluk...

Immunodominant antigens of an approximate molecular mass of 27 kD were obtained from an excretory-secretory product of adult Fasciola gigantica by a continuous-elution method. An indirect ELISA using the antigens obtained by this relatively simple procedure was developed for detecting specific antibodies from patients infected with F. gigantica. Sera from patients with other parasitic infection...

Fatty acid binding protein (FADP) is an attractive vaccine candidate and a drug target for controlling fasciolosis caused by Fasciola gigantica in ruminants. The cDNA encoding F. gigantica FADP was amplified by polymerase chain reaction and expressed in Escherichia coli as a recombinant protein using a prokaryotic expression system. The protein was purified to homogeneity by nickel chelating af...

following the occurrence of two large outbreaks in gilan province, northern iran, the public health importance of human fascioliasis has increased significantly. the mixed infections of fasciola gigantica and f. hepatica within a given domestic animal individual suggest a very complicate picture of possible circulation ways of the parasite and the possibility for humans to be infected with both...

The purpose of the present study was to evaluate the potential role of the 27-Kilodalton (KDa) antigen versus Fasciola gigantica adult worm regurge antigens in a DOT-Blot assay and to assess this assay as a practical tool for diagnosis fascioliasis in Egyptian patients. Fasciola gigantica antigen of an approximate molecular mass 27-(KDa) was obtained from adult worms by a simple elution SDS-PAG...

Indonesian thin-tail (ITT) sheep resist infection by Fasciola gigantica by an immunological mechanism within 2 to 4 weeks of infection yet are susceptible to F. hepatica infection. Studies of ITT sheep show that little liver damage occurs following F. gigantica infection, suggesting that the invading parasites are killed within the peritoneum or shortly after reaching the liver. We investigated...

BACKGROUND Fascioliasis, caused by Fasciola hepatica and F. gigantica, has medical and economic importance in the world. Molecular approaches comparing traditional methods using for identification and characterization of Fasciola spp. are precise and reliable. The aims of current study were molecular characterization of Fasciola spp. in West Azerbaijan Province, Iran and then comparative analys...

Over the last decade, diagnostic tools to detect and differentiate Fasciola species have improved, but our understanding of the distribution of haplotypes and population structure of this parasite is less clear. This study was designed to survey this gap in the F. gigantica epidemiology in Kermanshah province, western Iran from 2015 to 2017.Sixty-eight Fasciola isolat...