Circulating immune complexes were investigated by the 125I-Clq binding test in serum from patients with fascioliasis. Only 36% of all the patients studied showed significant levels of CIC. Nevertheless, when we considered only the patients eliminating Fasciola hepatica eggs in the stool and/or with the acute phase of the infection, the detection of CIC was very higher (more than 70% of the case...

Understanding genetic structure and status of genetic variation of the Fasciola gigantica and F. hepatica isolates from different hosts, has important implications for epidemiology and effective control of fasciolosis. The aim of the present work was to study the molecular characterization of Fasciola gigantica and F. hepatica isolates collected from cows and sheep, using the random amplified p...

A single-step multiplex PCR (here referred to as a duplex PCR) has been developed for simultaneous detection and diagnosis of Fasciola hepatica and F. gigantica. These species overlap in distribution in many countries of North and East Africa and Central and Southeast Asia and are similar in egg morphology, making identification from fecal samples difficult. Based on a comparative alignment of ...

BACKGROUND Fasciola species are the main causes for fascioliasis with great financial losses and are among the most important food/water-borne parasites worldwide. The basic proceedings such as epidemiology and effective control of fascioliasis rely mainly on precise identification of Fasciola species. The present study was conducted to determine the Fasciola species in ruminant fecal samples f...

background: fascioliasis is a chronic hepatic disease and may be resulted from mechani­cal/molecular parasite adhesion to host liver tissue. the aim of this study was to detect surface car­bohydrate and lectin, carbohydrate-binding protein isolation that might be responsible of this molecular binding. methods: the present experimental work was conducted in the department of medical parasitol­og...

AIM Fascioliasis is one of the most zoonotic diseases with global extension. As the epidemiological distribution of Fasciola may lead to various genetic patterns of the parasite, the aim of this study is to identify Fasciola hepatica based on spermatogenesis, and phylogenetic analysis using mitochondrial (nicotiamide adenine dinucleotide dehydrogenase subunit I [ND1] and cytochrome oxidase subu...

The aim of this study was to identify Fasciola isolates collected from natural livers of livestock by RFLP and sequencing in southwest of Iran. Morphological examination and biologic characters cannot cause a certainty in the accurate and precise identification and intra and inter-specific differences of Fasciola spp. In this study, we identified Fasciola species using 28S marker in isolates fr...

BACKGROUND In this study, we used both ITS1 and ITS2 for molecular identification of Fasciola species. METHODS The region between 18S and 28S of ribosomal DNA was used in PCR-RFLP method for molecular identification of Fasciola species. Ninety trematodes of Fasciola were collected during abattoir inspection from livers of naturally infected sheep and cattle from Khorasan, East Azerbaijan, and...

Fasciola hepatica, a liver fluke, is a causative agent of fascioliasis in mammals (sheep, cattle, goat, ox and other ruminants, pig, hare rabbit, beaver, elephant, dog, cat and kangaroo). In the unusual hosts, such as man and the horse, the fluke may be found in the lungs, under skin or other situations. The parasite Fasciola hepatica is a hermaphroditic trematode, which is particularly common ...