BACKGROUND Medium chain C8-C10 triglycerides (MCTs) improve fat absorption in short bowel patients. Effects on overall energy absorption remain unknown. AIMS To determine whether MCTs and medium chain fatty acids (MCFAs) are absorbed in the colon like the short chain fatty acids (SCFAs) or are lost in faeces similarly to long chain fatty acids (LCFAs). METHODS Nine small bowel resected pati...

We investigated the effect of the A-IV-2 allele, which encodes a Q360H substitution in apolipoprotein (apo) A-IV, and dietary fat on cholesterol absorption in humans. In three separate studies we compared fractional intestinal cholesterol absorption between groups of subjects heterozygous for the A-IV-2 allele (1/2) and homozygous for the common allele (1/1) receiving high cholesterol ( approxi...

OBJECTIVE Quantitative balance studies were performed to compare fat and calcium absorption in healthy, full term infants fed casein hydrolysate-based (CHF) and soy protein-based (SPF) infant formulas with or without palm olein (PO). Previous studies have reported that PO significantly reduced absorption of both fat and calcium in cow's milk-based formulas in which most of the calcium is inhere...

Absorption of dietary fat in the small intestine is accompanied by a rise of intestinal alkaline phosphatase (IAP) in the serum and of secretion of IAP-containing surfactant-like particles from the enterocytes. In the present work, fat absorption was studied in organ cultured mouse intestinal explants. By immunofluorescence microscopy, fat absorption caused a translocation of IAP from the enter...

This study generated pancreatic triglyceride lipase (PTL)-null mice to test the hypothesis that PTL-mediated hydrolysis of dietary triglyceride is necessary for efficient dietary cholesterol absorption. The PTL-/- mice grew normally and displayed similar body weight as their PTL+/+ littermates. Plasma lipid levels between animals of various PTL genotypes were similar when they were maintained o...

There has been a recent resurgence of interest in bile salt metabolism. Normally the body contains about 3 g. of bile salts. These comprise conjugates of cholic acid and chenodesoxycholic acid. The free acids are derived from the oxidation of cholesterol in the liver and once formed are conjugated with either taurine or glycine, after which they are secreted into the bile and so enter the small...

background: fat-rich diet may alter oocyte development and maturation and embryonic development by inducing oxidative stress (os) in follicular environment. objective: to investigate the relationship between fat intake and oxidative stress with oocyte competence and embryo quality. materials and methods: in observational study follicular fluid was collected from 236 women undergoing assisted re...

OBJECTIVE Quantitative determination of fecal fat still is the gold standard for measuring malabsorption. We evaluated the importance of standardized food intake before and under the collection of feces. MATERIAL AND METHODS In a project, evaluating patients with suspected chronic pancreatitis (CP) and healthy volunteers (HC), stools were collected for 72 hours coupled to registration of nutr...

In studies in animals it has been shown (1) that the chain length of a fatty acid is related to the extent of its absorption; i.e., the greater the molecular weight, the less the degree of absorption. It has been our purpose to find whether this relationship is true also in patients with steator-rhea. We have studied the absorption of various fatty acids, fed as triglycerides, in patients who h...