cryopreservation is the method of choice for long term storage of human pbmcs. this study was designed to compare the different combinations of variables affecting the cryopreservation of pbmcs samples. the viability of pbmcs separated from 2×5 ml peripheral blood samples obtained from 16 healthy adult volunteers, were measured using trypan blue dye exclusion method just before freezing with di...

PURPOSE To compare cryopreserved human corneal endothelial cells (HCECs) grown in human serum-supplemented media (HS-SM) with cryopreserved HCECs grown in fetal bovine serum-supplemented media (FBS-SM). METHODS Three pairs of human corneas from donors aged 8, 28, and 31 years were obtained from the eye bank. From each pair, one cornea was used to start a HCEC culture using HS-SM; the other co...

Fetal bovine serum (FBS) is a common component of animal cell culture media. It is harvested from bovine fetuses taken from pregnant cows during slaughter. FBS is commonly harvested by means of a cardiac puncture without any form of anaesthesia. Fetuses are probably exposed to pain and/or discomfort, so the current practice of fetal blood harvesting is inhumane. Apart from moral concerns, sever...

In this work we study discrete analogues of an exact sequence vector bundles introduced by M. Atiyah in 1957, associated to any smooth principal $G$-bundle $\pi:Q\rightarrow Q/G$. the original setting, splittings correspond connections on bundle $\pi$. The that consider here can be studied two different categories: category fiber with a (chosen) section, FBS, and local Lie groupoids, lLgpdC. FB...

BACKGROUND Cardiotocography (CTG) is worldwide the method for fetal surveillance during labour. However, CTG alone shows many false positive test results and without fetal blood sampling (FBS), it results in an increase in operative deliveries without improvement of fetal outcome. FBS requires additional expertise, is invasive and has often to be repeated during labour. Two clinical trials have...

Dear Editor, Soft tissue foreign bodies (FBs) are a common occurrence in emergency departments. Not all FBs are discovered during the initial patient encounter; several signs reveal the presence of a retained FB in a wound.1 Some FBs cause significant problems, including infl ammation, chronic pain and repeat visits, whereas some FBs are asymptomatic and remain undetected for months or years. W...

Myrmecophytic Acacia species produce food bodies (FBs) to nourish ants of the Pseudomyrmex ferrugineus group, with which they live in an obligate mutualism. We investigated how the FBs are protected from exploiting nonmutualists. Two-dimensional gel electrophoresis of the FB proteomes and consecutive protein sequencing indicated the presence of several Kunitz-type protease inhibitors (PIs). PIs...

We developed a novel thermal spraying technology for a silver-containing hydroxyapatite (Ag-HA) coating with antibacterial activity to reduce the incidence of implant-associated infections. In this study, we determined the concentration of Ag ions that show antibacterial activity in fetal bovine serum (FBS) and antibacterial activity of the Ag-HA coating in FBS. The minimum inhibitory concentra...

Two channel lter banks (FBs) were developed for subbandspeech coding [16]. Since then FB theory has grown to includeFBs with more than two channels, tree-structured FBs etc. Arich theory for the design and implementation of FBs exists.Wavelets were originally developed as an alternative to theSTFT for non-stationary signal analysis [26]. Since then it hasbeen realized th...

Bovine viral diarrhea virus (BVDV) isolation and antibody survey were performed using 2,758 fetal bovine sera (FBS) collected from slaughterhouses in New Zealand, Australia and the Dominican Republic, and then sent to Japan to manufacture commercial serum for cell culture use. FBS in the Dominican Republic were pooled for each several individuals, and those collected in other countries were sep...