Methods. Forty-four surgically excised melanocytic lesions of the iris were histologically classified as nevus or melanoma. Morphometric analysis using a digital filar micrometer (LaSICO 1602N-10 and 5-4A) measured the mean size of the 10 largest nucleoli, and Feulgen staining and image cytometry (CAS 200 Cell Analysis Systems) analyzed the nuclear DNA ploidy in the lesions. Patient follow-up i...

Reports of changes in DNA content of certain types of cells following exposure to conditions of stress has led to the suggestion that two kinds of DNA may be present. One is genetic DNA, and the other is called "metabolic" DNA. In a further attempt to investigate the possibility of this phenomenon, determinations of DNA content were made on Feulgen-stained nuclei of adrenal glands and kidneys i...

OBJECTIVE To examine the cytoprotective effect of melatonin or recombinant human growth hormone (hGH) on the early phase of a running myocardial infarction in rats by using the Feulgen staining. METHODS Rats were subjected to surgical ligature of the left coronary artery or its sham-operation and were studied 1.5 3 h later. Melatonin was administered in the drinking water (100 microg/ml water...

The structure and mode of division of the nucleus of budding yeast cells have been studied by phase-contrast microscopy during life and by ordinary microscopy after Helly fixation. The components of the nucleus were differentially stained by the Feulgen procedure, with Giemsa solution after hydrolysis, and with iron alum haematoxylin. New information was obtained in cells fixed in Helly's by di...

PURPOSE To compare the histologic, morphometric and nuclear DNA content of a group of benign and malignant melanocytic lesions of the iris. METHODS Forty-four surgically excised melanocytic lesions of the iris were histologically classified as nevus or melanoma. Morphometric analysis using a digital filar micrometer (LaSICO 1602N-10 and 5-4A) measured the mean size of the 10 largest nucleoli,...

Specimens of cells derived from tumors of the human female genital tract plus normal cells as standards have been divided into aliquots and stained according to acridine orange or pararosanilin:Feulgen procedures. Acridine orange-stained cells were slit-scanned for 535 nm nuclear fluorescence; Feulgen-stained cells were comb-scanned for 580 nm nuclear absorbance. For each specimen examined, the...

Background: Different histochemical stains have been applied to demonstrate the cytotoxic and genotoxic effects of cigarette smoking on cells. Feulgen Papanicolaou were most popular nuclear abnormalities. The aim this study was compare in demonstrating exfoliated oral mucosa Methods: A total 31 smokers 15 non-smokers included case-control study. Using a wooden spatula, two samples taken from ea...

Breakthroughs in microscopy technology provide new insights into cell biology. Early microscopes allowed Robert Hooke to see cells. Improved staining techniques enabled Camillo Golgi to see the apparatus that bears his name, and Robert Feulgen to visualize DNA in chromosomes. Similarly, EM allowed Keith Porter to visualize the endomembrane system. More recently, transgenic technology using fluo...

Of all substances which bulk large in the chemical composition of cellular structures, desoxypentose nucleic acid is perhaps the most clearly demonstrable by application of cytochemical methods to cells. For each one of the three components of nucleotide residues there is a specific method which -enables the cytologist to visualize it on slides: the purines and pyrimidines have natural specific...