Introduction: Oocyte quality and fertility decline with advanced maternal age. During maturation within the ovarian follicle, oocyte relies on associated somatic cells, specifically cumulus granulosa to acquire essential components for developmental capacity. Methods: A nontargeted metabolomics approach was used investigate effects of mare age different cell types dominant, follicular-phase fol...

BACKGROUND A growing body of evidences suggests that the ovary is a site of inflammatory reactions, and thus, ovarian cells could represent sources and targets of the interleukin-1 (IL-1) system. The purpose of this study was to examine the IL-1 system gene expressions in equine granulosa cells, and to study the IL-1beta content in follicular fluid during the follicle maturation. For this purpo...

BACKGROUND Egg laying in Magang geese is characterized by extended interruption between clutches and lowing laying rate. Both the ovarian follicular development and ovulation characteristics, and the associated endocrine and molecular regulatory mechanisms involved are poorly understood, but could be important for guiding development of molecule aided selection of egg laying performances in gee...

Background: Ovarian tissue cryopreservation and autotransplantation is the only fertility preservation way for prepubertal girls and situations that cytotoxic treatment is urgent. Materials and Methods: The ovaries of premature (5 weeks old) rats were autografted under cervical skin after vitrification. 3 weeks later, transplanted ovaries were removed from the body and structure of ovary, expre...

Background:Differentiation of Embryonic Stem Cells into Oocyte-like cells in vitro is challenging. Successful derivation of oocyte from stem cells can provide an alternative source for curing ovogenesis problems. The current study aims to demonstrate a new protocol with two different types of media for differentiating embryonic stem cells (ESCs) into oocyte-like cells ...

The objective of this study was to compare pregnancy rates in domestic cats using fresh semen for the bilateral intrauterine insemination (BIUI) method and novel uterine body (UBI) method. Queens received a single injection equine chorionic gonadotropin (eCG) (200 IU; IM) induce ovarian follicular development and, after 83 h, an human (hCG) (100 final oocyte maturation ovulation induction. Thir...

Ovarian follicular fluid contains both stimulatory and inhibitory agents that influence the growth and maturation of oocyte. In the present study, an attempt was made to isolate and study the biological properties of ovarian follicular fluid peptide(s) in buffaloes. Bubaline ovarian follicular was made steroidand cell-free. A protein fraction was obtained by saturation (30-35% level) of the fol...