Background: Generation of an effective dendritic cell (DC) based cancer vaccine depends on appropriate differentiation of monocytes in vitro. Objective: To compare the effects of monocyte separation methods, flask adherence (Flask-DC) and magnetic activated cell sorting (MACS-DC), on phenotypic and functional characteristics of resultant DCs. Methods: DCs from healthy volunteers were generated ...

background: fibroblasts are one of the important cells in wound healing. these cells create a proper bed for keratinocytes migration and wound contraction.wound healing in distal limb of horses has complications, such as formation of exuberant granulation tissue (egt). the main factor in this problem is overgrowing of fibroblasts. objectives: the purpose of the present study was to compare fibr...

This study aimed to evaluate the effect of flask types, post-pressing times and base regions on the base adaptation of maxillary and mandibular complete dentures. Denture bases were waxed in stone casts and randomly divided into six groups (n=10), according to conventional and double flasks inclusion, and immediate and 6 hours post-pressing times. Denture bases were polymerized in water bath at...

The spinner flask bioreactor has been widely used in in vitro cell culturing processes due to its superiority in providing a homogeneous culture environment compared to traditional culturing methods. However, there is limited understanding of the flow fields in these bioreactors, and optimum culture conditions are yet to be determined. This article presents the experimental characterization of ...

Materials with low thermal conductivity like aerogel, acrylic, aluminum silicate, superfine glass wool, and phenolic foam are often used as insulation materials in engineering have noticed increasing applications other spheres of life, buildings vacuum flasks. The objective this research is to study the temperature variation coffee over 10 hours a selected flask while varying geometrical materi...

2. Soxhlation: Soxhlet extraction is only required where the desired compound has a limited solubility in the solvent, and the impurity is insoluble in that solvent. If the desired compound has a high solubility in a solvent then a simple filtration can be used to separate the compound from the insoluble substance [1]. In a conventional soxhlet system plant material is placed in thimble-holder,...

background: generation of an effective dendritic cell (dc) based cancer vaccine depends on appropriate differentiation of monocytes in vitro. objective: to compare the effects of monocyte separation methods, flask adherence (flask-dc) and magnetic activated cell sorting (macs-dc), on phenotypic and functional characteristics of resultant dcs. methods: dcs from healthy volunteers were generated ...

Embryonic stem (ES) cells can differentiate into functional hepatic lineage cells, which can potentially be used in biomedicine. To obtain hepatic lineage cells from ES cells, embryoid bodies (EBs) must be formed. In this study, we developed an EB formation system using a spinner flask for mass production of EBs. ES cells were inoculated into the spinner flask, where they formed EBs within 4 d....

Cellulase production study was performed in shake flask and bioreactor system using Cellulomonas cellulans NRRL B 4567 for initial substrate concentration from S0 = 2 to 12 g L . The growth, substrate uptake profile and enzyme activity at different initial substrate concentrations were measured. The results inferred the presence of substrate inhibition kinetics. Various substrate inhibition mod...