Because it has been difficult to identify and separate malignant cells in human lymphoid malignancies, we have developed a flow cytometry-based fluorescent in situ hybridization (FISH) technique using immunoglobulin (Ig) heavy chain variable region (V H) gene probes. After obtaining the specific V H gene sequence expressed by the multiple myeloma IM-9 cell line and the malignant cells in five m...

The applications of flow cytometry are being extended beyond cells into molecular interactions and genomic analyses. The authors explain how instrumentation and reagent development are combining to improve flow cytometric throughput by orders of magnitude beyond that possible just a few years ago, such that the combination of sensitivity, throughput and versatility makes flow cytometry an analy...

This study presents results of histopathologic, cytogenetics and flow cytometry analyses performed on Macoma balthica collected from the Gulf of Gdansk (Baltic Sea) in 2003 in order to compare the techniques for diagnosis of neoplasia. The proportion of affected clams gave a crude prevalence of 15.7 %. The four stages of the disease defined by histology and three stages of neoplasia defined by ...

background: ferritin is an iron storage protein, which plays a key role in iron metabolism. measurement of ferritin level in serum is one of the most useful indicators of iron status and also a sensitive measurement of iron deficiency. monoclonal antibodies may be useful as a tool in various aspects of ferritin investigations. in this paper, the production of a murine monoclonal antibody (mab) ...

background: gp41 of hiv (human immunodeficiency virus) is a protein that mediates fusion between viral and cellular membranes. the agent, t-20, which has been approved for hiv inhibition, can restrain gp41 function in the fusion process; nevertheless, it has disadvantages like instability, high cost of production and injection form to be delivered twice a day. methods: several molecules like nb...

immunophenotyping of lymphocytes is very essential for evaluation of immune system. due  to  the  effect  of  environmental  factors  and  ethnical diversity on  immune  system, establishment of an internal normal range of lymphocyte subsets is a necessity for each population.  the  aim  of  this  study  was  to  determine  the  normal  range  of  t  and  b lymphocytes, and nk cells in normal i...

in our search for new anticancer medicinal plants, the antiproliferative activity of the methanol extract of daphne mucronata (thymelaeaceae) was evaluated using human myelogenous leukemia k562 cells. the cells responded to plant treatments in a dose dependent manner and the ic50 of the crude extract (equivalent to 1 g of plant leaves powder per ml) and the purified active component were found ...