نتایج جستجو برای: fluorescence in situ hybridization

تعداد نتایج: 17000389  

Journal: :genetics in the 3rd millennium 0
رضوان حبیبیان rezvan habibian genzyme genetics, pasadena, ca جودیت ناپس آتیه حاجیان پور

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Journal: :avicenna journal of medical biotechnology 0

background: various fixation and permeabilization techniques have been developed for detection of intracellular antigens by flow cytometry; however, there are few studies using flow cytometry to detect the frequency of intracellular nucleic acids, particularly rna. we tested six different permeabilization methods in order to gain access to a high quality method with minimal damage to intracellu...

2010
Emel Ebru PAlA Emel Ebru PALA Murat KAPKAç

Objective: HEr2/neu is overexpressed/amplified in 20% of breast cancers. HEr2/neu status plays a role in determining the patients who might benefit from hormonal therapy and targeted therapy with Trastuzumab. The main cause of HEr2/neu overexpression is gene amplification. 10-25% of patients show Topoisomerase II-alpha gene alterations with HEr2/neu amplification. The objective of this study wa...

Journal: :Journal of Korean Medical Science 1999
J. I. Kim J. H. Rhee

Major aneuploidies diagnosed prenatally involve the autosomes 13, 18, and 21, and sex chromosomes. Fluorescence in situ hybridization (FISH) allows rapid analysis of chromosome copy number in interphase cells. The purpose of this study was to evaluate the role of multicolor fluorescence in situ hybridization in simultaneous detection of probe sets for chromosome 18, X, and Y in uncultured amnio...

Journal: :Nucleic acids research 2004
Yan Xiao Peter E Barker

To improve signal stability and quantitation, an optically stable, novel class of fluorophore for hybridization analysis of human metaphase chromosomes is demonstrated. Detection of hybridization sites in situ was based on fluorescence from streptavidin-linked inorganic crystals of cadmium selenide [(CdSe)ZnS]. Fluorescence of nanocrystal fluorophores was significantly brighter and more photost...

Journal: :Journal of Histochemistry & Cytochemistry 1993

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