We have created a novel fluorescent model of a human ovarian carcinoma xenograft overexpressing receptor HER2, a promising molecular target of solid tumors. The model is based on a newly generated SKOV-kat cell line stably expressing far-red fluorescent protein Katushka. Katushka is most suitable for the in vivo imaging due to an optimal combination of high brightness and emission in the "windo...

Phenazines, a group of fluorescent small molecules produced by the bacterium Pseudomonas aeruginosa, play a role in maintaining cellular redox homeostasis. Phenazines have been challenging to study in vivo due to their redox activity, presence both intra- and extracellularly, and their diverse chemical properties. Here, we describe a noninvasive in vivo optical technique to monitor phenazine co...

Various bacteria were isolated from the casing layer soil of the culture bed of P. ostreatus and their role in fruiting body induction of the edible mushroom, P. ostreatus, was investigated. Analysis of the bacterial community isolated from the casing layer soil revealed that the composition of genera and number of cultivable bacteria were different for each sterilizing treatment. Bordetella wa...

Pseudomonas cepacia was found to be resistant to the outer membrane-permeabilizing effects of aminoglycoside antibiotics, polymyxin B, and EDTA. Permeabilization of P. cepacia to the fluorescent probe 1-N-phenylnaphthylamine was not achieved at concentrations 100- to 1,000-fold above those required to permeabilize Pseudomonas aeruginosa. Furthermore, in contrast to P. aeruginosa cells, intact c...

Root formation and root architecture of olive microshoots, inoculated or not with Pseudomonas fluorescent P19 or P21, were evaluated by measuring of length and the numbers of adventitious and lateral roots. Three-four nodal in vitro shoots were treated with different population densities (0, 105, 108 CFUml-1) of rhizobacteria. The density of 108 CFUmgl-1 induced almost two times increase in num...

The spatial organization of cells within bacterial aggregates on leaf surfaces was determined for pair-wise mixtures of three different bacterial species commonly found on leaves, Pseudomonas syringae, Pantoea agglomerans, and Pseudomonas fluorescens. Cells were coinoculated onto bean plants and allowed to grow under moist conditions, and the resulting aggregates were examined in situ by epiflu...

Indiscriminate use of chemicals as fertilizers and pesticides gives rise to substantial harm the environment ecosystem along with animals humans. More importantly, replace such hazardous agrochemicals, many biological solutions are available in nature which is present form microorganisms having capacity promote plant growth substantially. Pseudomonas play a major role plants like inducing syste...

A novel strategy termed habitat-inducible rescue of survival (HIRS) was developed to identify genes of Pseudomonas syringae that are induced during growth on bean leaves. This strategy is based on the complementation of metXW, two cotranscribed genes that are necessary for methionine biosynthesis and required for survival of P. syringae on bean leaves exposed to conditions of low humidity. We c...

Interactions between plants and microorganisms in the rhizosphere (rhizobacteria) can clearly affect crop yields. Rhizobacteria that benefit plant growth and development are called PGPR. the most studied PGPR belong to gramnegative genera, and the greatest number of strains are members of the fluorescent pseudomonads in our present investigation was to study the plant growth promoting (PGP) act...