From the Department of Ophthalmology, Biochemistry Research Laboratory (The Sir Isaac and Lady Wolfson Ophthalmic Research Laboratories), Hebrew University-Hada.ssah Medical School, Jerusalem, Israel. 1 Present address: Department of Biophysics, Weizmann InstituteofScience,Rehovot,Israel. Nonstandard abbreviations used: MPS, mucopolysaccharides (glycosaminoglycans),mucopolysaccharidoses (with r...

We describe a simple, noninstrumental procedure for detecting hemoglobin S in samples of blood on filter paper, with use of small volumes of reagents. The method depends on the differential solubilities of reduced sickling and nonsickling hemoglobin in phosphate solutions (1, 2) or Sickledex reagent’ and the apparent solubilizing effect of urea on reduced hemoglobin S (3). The color of paired p...

dna microarray is a powerful approach to study simultaneously, the expression of 1000 of genes in a single experiment. the average value of the fluorescent intensity could be calculated in a microarray experiment. the calculated intensity values are very close in amount to the levels of expression of a particular gene. however, determining the appropriate position of every spot in microarray im...

This protocol describes a method to image individual mRNA molecules in situ. About 50 oligonucleotides complementary to different regions of a target mRNA species are used simultaneously. Each probe is labeled with a single fluorescent moiety. When these probes bind to their target, each mRNA molecule becomes so intensely fluorescent that it can be seen as a fine fluorescent spot. Several diffe...

In food microbiology, Rambach agar facilitates the differentiation of non-typhi Salmonella through a specific red pigmentation of the colonies. The usefulness of Rambach agar was examined relative to its usefulness to the field of clinical microbiology. Of 170 non-typhi Salmonella strains, 92 and 97% gave bright red colonies after 24 and 48 h of incubation, respectively, while 100% of 112 other...

Kovacs indole reagent, p-dimethylaminobenzaldehyde, Ehrlich indole reagent and p-dimethylaminocinnamaldehyde were used as spot indole reagents to test 359 strains of gram-negative rods growing on 5% sheep blood agar, Trypticase soy agar (BBL Microbiology Systems), and MacConkey agar. The p-dimethylaminocinnamaldehyde reagent was the most sensitive of those tested and provided results that were ...