Methods Three groups of rats were considered: SHAM, Bile Duct Ligation (BDL), BDL + hyperammonic dietary (BDLNH3). In each group, rats were treated by BNa or RFX. HE was assessed using neurocomportemental testing (6 minutes tail suspension test assessing the time of immobility). NH3 levels were assessed before sacrifice. BBB permeability was assessed by IV injection of a fluorochrome (Texas Red...

Most proteins have not evolved for maximal thermal stability. Some are only marginally stable, as for example, the DNA-binding domains of p53 and its homologs, whose kinetic and thermodynamic stabilities are strongly correlated. Here, we applied high-throughput methods using a real-time PCR thermocycler to study the stability of several full-length orthologs and paralogs of the p53 family of tr...

24 Background 25 Detecting serum antibody against inhaled antigens is an important diagnostic adjunct for 26 hypersensitivity pneumonitis (HP). We sought to validate a quantitative fluorimetric 27 assay testing serum from bird fanciers. 28 Methods 29 Antibody activity was assessed in bird fanciers and control subjects using various avian 30 antigens and serological methods, and the titer was co...

BACKGROUND In animal models of endometriosis, the identification and quantification of lesions originating from human endometrium is often hampered by the small size of the implants and their embedding in murine tissue. The purpose of the present study was to develop two new methods of quantifying endometriosis-like lesions in a nude mouse model: fluorimetry and morphometry. METHODS Human men...

Simple, quantitative assays to measure pH in tissue could improve the study of complicated biological processes and diseases such as cancer. We evaluated multispectral fluorescence imaging (MSFI) to quantify extracellular pH (pHe) in dye-perfused, surgically-resected tumor specimens with commercially available instrumentation. Utilizing a water-soluble organic dye with pH-dependent fluorescence...

BACKGROUND Detecting serum antibody against inhaled antigens is an important diagnostic adjunct for hypersensitivity pneumonitis (HP). We sought to validate a quantitative fluorimetric assay testing serum from bird fanciers. METHODS Antibody activity was assessed in bird fanciers and control subjects using various avian antigens and serological methods, and the titer was compared with symptom...

24 Background 25 Detecting serum antibody against inhaled antigens is an important diagnostic adjunct for 26 hypersensitivity pneumonitis (HP). We sought to validate a quantitative fluorimetric 27 assay testing serum from bird fanciers. 28 Methods 29 Antibody activity was assessed in bird fanciers and control subjects using various avian 30 antigens and serological methods, and the titer was co...

Carbachol-evoked rises in [Ca2+]i were measured in fura-2-loaded, rat parotid acinar cells. In suspensions of dissociated cells examined by dual wavelength excitation fluorimetry, a maximally effective concentration of carbachol produced a measured peak [Ca2+]i of 780 +/- 60 nM followed by a maintained elevation in the presence of 1 mM external Ca2+, and a peak of 630 +/- 95 nM followed by a re...

OBJECTIVES To develop and optimize quantitative HPLC method using 2,3-naphthalenedicarboxaldehyde (NDA) after simple and efficient solid phase extraction to determine the histamine in a biopharmaceutical (Histobulin™). METHODS The HPLC method was established using NDA-induced Histobulin and compared with the recently reported HPLC method using o-phthaldehyde (OPA). The validated NDA-applied H...

Several putative roles for ryanodine receptors (RyR) were investigated in human and bovine airway smooth muscle. Changes in intracellular Ca2+ concentration ([Ca2+]i) and membrane current were investigated in single cells by confocal fluorimetry and patch-clamp electrophysiology, respectively, whereas mechanical activity was monitored in intact strips with force transducers. RyR released Ca2+ f...