OBJECTIVE To determine whether serum fructosamine correlates with glycemic control and clinical outcomes in patients being screened for cystic fibrosis-related diabetes (CFRD). METHODS Fructosamine and percent predicted forced expiratory volume in 1s (FEV1) were measured in patients undergoing a 2h oral glucose tolerance test (OGTT) for CFRD screening. Fractional serum fructosamine (FSF) was ...

The in vivo formation of fructosamines following non-enzymatic reaction of proteins with glucose (i.e. glycation) was first described almost 30 years ago. Until recently, the only known fate of fructosamines in mammalian cells was their spontaneous conversion into advanced glycation end products. The identification in human erythrocytes of a new enzyme, fructosamine 3-kinase, disclosed the exis...

We compared the fructosamine activity in sera from healthy and diabetic subjects with the degree of protein glycation detected by a liquid-chromatographic method. The latter technique measures furosine as a specific product after hydrolysis of epsilon-amino-fructose-lysine. Our results indicate that the fructosamine assay measures the extent of glycation of purified human serum albumin correctl...

The fructosamine test for assessing control of glucose in blood has been extensively evaluated, but some questions remain regarding its validity. From the analytical and clinical evaluation we present here, we conclude that: the test is sensitive to variations in the composition of the sample protein; the fructosamine reaction is almost completely unaffected by labile fractions; the concentrati...

A commercial kit fordetermining fructosamine was evaluated. The reference interval (determined from data on 183 nondiabetic subjects) was 1.67 to 2.85 (mean 2.17) mmol/L. Serum and plasma (EDTAor heparin-anticoagulated) gave equivalent results; plasma treated with fluoride/oxalate gave slightly lower values. The between-run CV was <4%. Fructosamine values were similar by the present method and ...

We have calibrated a secondary serum protein standard by use (as primary standards) of samples of albumin and polylysine glycated with [14C]glucose in vitro, the glycation of which was assessed by radioactivity measurements and by elementary analysis for C and N. Using this standard for calibration in our improved fructosamine assay, one obtains an average fructosamine value of 247 mumol/L for ...

AIM To determine whether the Ramadan fasting can improve metabolic control evaluated from serum fructosamine and beta hydroxybutirate in patients with Type 2 diabetes mellitus. METHODS This was a prospective one group before and after study (self-controlled study). Twenty four patients from the outpatient clinic of the Metabolic Endocrinology Division of the Department of Internal Medicine, F...

An 8-year-old male Austrian Pinscher and a 14-year-old male Golden Retriever were presented for evaluation due to unexplainable high fructosamine values despite euglycemia and epistaxis in combination with polydipsia/polyuria, respectively. Blood analysis revealed severe hyperglobulinemia, hypoalbuminemia and markedly elevated fructosamine concentrations in both dogs. Multiple myeloma with IgA-...

We investigated the ability of the fructosamine assay to detect glycation of serum proteins. We incubated both whole human serum and serum protein fractions in vitro with [14C]glucose, and analyzed for reducing activity and for uptake of 14C by protein. In all experiments, the reducing activity increased linearly with time for seven days and was correlated with 14C uptake (r = 0.94-0.98). Prote...

Aims HbA1c can usually be used as the indicator of glucose control and correlated with the development of long-term diabetic complications. But there are limits that it usually reflects the mean glucose levels of 2-3 months and can be variable in the situation of hemoglobinopathy or the conditions of altered RBC lifespan. In contrast, serum fructosamine levels reflect the mean glucose levels of...