Fourier transform infrared (FTIR) microspectroscopy has been applied to a study of prostate cancer cell lines derived from different metastatic sites and to tissue from benign prostate and Gleason-graded malignant prostate tissue. Paraffin-embedded tissue samples were analysed by FTIR, after mounting onto a BaF(2) plate and subsequent removal of wax using Citroclear followed by acetone. Cell li...

H2O diffusion in a haploandesitic melt (a high-silica and Fe-free andesitic melt, NBO/T = 0.173) has been investigated at 1 GPa in a piston-cylinder apparatus. We adopted a double diffusion couple technique, in which one couple was composed of a nominally anhydrous glass with 0.01 wt.% H2O and a hydrous glass with 5.7 wt.% H2O, and the other contained the same nominally anhydrous glass and a hy...

Evaluation with FTIR +Turunen, M J; Jurvelin, J S; Kröger, H; Isaksson, H +University of Eastern Finland, Kuopio, Finland, Kuopio University Hospital, Kuopio, Finland, Lund University, Lund, Sweden [email protected] INRODUCTION: Bone structure and composition adapt to its mechanical loading environment [1] and show variations with age [2,3]. Trabecular bone is highly metabolically active, a...

In Fourier transform infrared (FTIR) microspectrocopy, the tissue preparation method is crucial, especially how the tissue is cryo-sectioned prior to the imaging requires special consideration. Having a temperature difference between the cutting blade and the specimen holder of the cryostat greatly affects the quality of the sections. Therefore, we have developed an optimal protocol for cryo-se...

The changes in chemical composition of human articular cartilage (AC) caused by osteoarthritis (OA) were investigated using Fourier transform infrared microspectroscopy (FTIR-MS). We demonstrate the sensitivity of FTIR-MS for monitoring compositional changes that occur with OA progression. Twenty-eight AC samples from tibial plateaus were imaged with FTIR-MS. Hyperspectral images of all samples...

An infrared beamline has been constructed at the 800 MeV electron storage ring, Aladdin. The beamline is located downstream of a short straight section, where it collects edge radiation produced by electrons exiting and entering the bending magnets at the ends of the straight section. Measurements at wavelengths of 1–5 μm suggest that the edge radiation is brighter than standard synchrotron rad...

Callus formation is a critical step for successful fracture healing. Little is known about the molecular composition and mineral structure of the newly formed tissue in the callus. The aim was to evaluate the feasibility of small angle x-ray scattering (SAXS) to assess mineral structure of callus and cortical bone and if it could provide complementary information with the compositional analyses...

Solanum lyratum is a medicinal plant used to treat cancers and tumors. The objective of this study was to apply Fourier transform infrared microspectroscopy (FTIR) and thermogravimetric analysis (TGA) to quick identification of S. lyratum fourteen samples from its main geographic origins in China. The hierarchical dendrogram based on principal component analysis (PCA) of FTIR and TGA data showe...

Synchrotron FTIR microspectroscopy was used to characterise and assess the chemical diversity of electrocoat primer, primer surfacer and basecoats of automotive paint samples from 75 vehicles representing a range of international car manufacturers. Significant diversity was found in the synchrotron FTIR data from the primer surfacer coats. Classification using principal component analysis revea...