Chlorinated pollutants are hardly biodegradable under oxic conditions, but they can often be metabolized by anaerobic bacteria through organohalide respiration reactions. In an attempt to identify bottlenecks limiting aerobic catabolism of 1,3-dichloroprop-1-ene (1,3-DCP; a widely used organohalide) in Pseudomonas pavonaceae, the possible physiological restrictions for this process were surveye...

Herein we report on the kinetic and protein expression of glucose-6-phosphate dehydrogenase (G6PDH), 6-phosphogluconate dehydrogenase, and malic enzyme (ME) in the liver of the trout ( Oncorhynchus mykiss) during a long-term starvation-refeeding cycle. Starvation significantly depressed the activity of these enzymes by almost 60%, without changing the Michaelis constant. The time response to th...

Background: Since the sheep ‘Dolly’ was successfully cloned by somatic cell nuclear transfer (SCNT), the inefficiency of SCNT cloning and abnormality of cloned animals have been a focus of considerable controversy. Oocyte quality has been shown to contribute to poor somatic cloning and cell reprogramming efficiency with many factors affecting oocyte quality and developmental potential. It has b...

We examined the effect of nitrate on the expression of the NADPH producing enzymes of the oxidative pentose phosphate pathway, glucose-6-phosphate dehydrogenase (G6PDH) and 6-phosphogluconate dehydrogenase (6PGDH) in maize seedlings (Zea mays L. W64A×A182E). In extracts of 5 day old maize roots and leaves treated with 10 mM KNO3, G6PDH and 6PGDH activities increased by 44 and 53%, respectively,...

An NADPH-dependent NO2--reducing system was reconstituted in vitro using ferredoxin (Fd) NADP+ oxidoreductase (FNR), Fd, and nitrite reductase (NiR) from the green alga Chlamydomonas reinhardtii. NO2- reduction was dependent on all protein components and was operated under either aerobic or anaerobic conditions. NO2- reduction by this in vitro pathway was inhibited up to 63% by 1 mm NADP+. NADP...

Dehydroepiandrosterone (DHEA) inhibits glucose 6-phosphate dehydrogenase (G6PDH) of different species and may potentially decrease intracellular glutathione. Therefore, it can have and enhance anti-parasitic action against Plasmodium spp. We evaluated the antiplasmodial activity and the interaction of DHEA with several antimalarial drugs. The inhibitory effect of DHEA on erythrocytic and G6PDH ...

The effect of different main dietary compositions on growth, anticipatory digestive enzyme activities, and oxidative status was studied in the proximal intestine juvenile European sea bass. A control diet (C, 44% protein, 17.6% lipid, 20% starch), three diets with increasing starch levels to test protein sparing (P36S36, P40S29, P43S24), two high lipid content (L20S13 L22S7) were tested. After ...

OBJECTIVE Ascorbic acid is a powerful antioxidant and protects against lipid peroxidation in cerebral membrane. The objective of the study was to investigate protective effects of ascorbic acid on lipid peroxidative damage and perturbation of enzymes, glucose-6-phosphate dehydrogenase (G6PDH) and acetylcholinesterase (AChE) in discrete regions of rat brain after vanadium exposure. METHODS Thi...

The effect of dietary gamma-hexachlorocyclohexane (lindane) (50-350 ppm, 0.17-1.19 mumol/kg chow) on the activity of enzymes of lipogenesis, viz., fatty acid synthase (FAS; EC 2.3.1.85), citrate cleavage enzyme (CCE; EC 4.1.3.8), malic enzyme (ME; EC 1.1.1.40), glucose-6-phosphate dehydrogenase (G6PDH; EC 1.1.1.49) and 6-phosphogluconate dehydrogenase (PGDH; EC 1.1.1.44), and on serum lipid lev...

Background: This study was conducted in order to determine whether BCB staining performed on immature bovine oocytes to assess G6pdhlevel has an impact on ivf/ivp technology in bovine embryos. Both spermatozoa and oocytes metabolize glucose through the pentose phosphate pathway (PPP), and NADPH appears necessary for gamete fusion. Activity of glucose 6-phosphate dehydrogenase (G6PDH), the key e...