Introduction The prediction of the function of a novel gene remains to be a challenging problem. Given a piece of coding sequence, one can deduce its function by finding homologous genes using sequence or protein structural alignment. One can also perform gene expression measurements or gene knockout experiments to determine the function of a gene. However, a gene may not have homology with any...

Description This data set contains cluster memberships for yeast genes clustered using fitness deficiency scores from gene knockout experiments from Giaever et al. Nature (2002). The 3000 most variable genes were clustered using k-means with 30 clusters Usage data(Phenoclusters) Format A matrix whose rows are the 3000 genes and whose two columns are gene name and cluster membership number. Refe...

Some insight into the developmental basis for imprinting specific genes during the evolution of mammals can be gained from conventional gene 'knockout' studies. However, the consequences of full loss of function are often wide-ranging and may obscure the critical, dosage-related phenotype. This review focuses on transgenic techniques employed to alter the dosage of imprinted genes, including th...

Understanding the biology of C. elegans relies on identification and analysis of essential genes, genes required for growth to a fertile adult. Approaches for identifying essential genes include several types of classical forward genetic screens, genome-wide RNA interference screens and systematic targeted gene knockout. Based on most estimates made from screening results thus far, from 15–30% ...

Cucurbits (Cucurbitaceae) include major horticultural crops with high nutritional and economic value that also serve as model plants for studying plant development crop improvement. Conventional breeding methods have made important contributions to the production of cucurbit but led a bottleneck because narrow genetic bases low variation rates these crops. With molecular techniques, innovations...

In any organism that reproduces sexually, DNA Recombination plays vital roles in the generation of allelic diversity as well as in preservation of genome fidelity. Genome fidelity is particularly important in plants because mutations occurring during the development of flowering plants are heritable and can be passed onto the next generation. One of the gene families that play crucial roles in ...

Genetically modified animals are used for industrial applications as well as scientific research, and studies on these animals contribute to a better understanding of biological mechanisms. Gene targeting techniques have been developed to edit specific gene loci in the genome, but the conventional strategy of homologous recombination with a gene-targeted vector has low efficiency and many techn...