there are many studies related to the production of a elisa kit for diagnosing virus infections. however, production of most kits depends on purification of whole virus particles, which involves the use of costly equipment and reagents. the purpose of this study was to check out if the anti-cp42 antibodies could be used as a diagnostic assay for detection of grapevine fanleaf virus (gflv). in t...

Grapevine fanleaf virus (GFLV) is one of a large class of plant viruses whose cell-to-cell transport involves the passage of virions through tubules composed of virus-encoded movement protein (MP). The tubules are embedded within modified plasmodesmata, but the mechanism of targeting of MP to these sites is unknown. To study intracellular GFLV MP trafficking, a green fluorescent protein-MP fusi...

An antiserum was raised against a fusion protein containing the C-terminal half of the protein (P3) encoded by the satellite RNA of grapevine fanleaf virus (GFLV; F13 isolate) and the N-terminal portion of the CI repressor of phage lambda. This antiserum specifically recognized P3 synthesized in the in vitro wheatgerm translation system and also in infected Chenopodium quinoa plants. In these p...

A pair of degenerate primers, GMPF1 and GMPR1, was designed on the basis of alignment of previously reported Grapevine fanleaf virus (GFLV) movement protein (MP) nucleotide sequences from Iran and other parts of the world. cDNA was synthesized by the use of Oligo d(T)18 from total RNA extraction from each diseased grapevine leaf sample and subjected to polymerase chain reaction (PCR) with the d...

Abstract Viral diseases in grapevine cause large economic losses due to decreased irregular yield and unbalanced ripening, can even lead plant mortality. There is a number of viral agents, few them have prominent impact their worldwide distribution, virulence, incidence. Although previous research has evaluated variations load between organs time since infection, there still lack knowledge on h...

to improve commercial grapevine cultivars, it is necessary to apply such methods that by cultivation of a certain number of vines in a certain area, higher yield could be harvested. to achieve such a goal, healthy stocks with resistant to pests and diseases, high fructification and high quality of vines are needed. all these properties can be obtained successfully through clonal selection. viti...

Changes in electric parameters of a mesoporous silicon treated by a plasma chemical etching with fluorine and hydrogen ions, under the adsorption of NEPO (Nematodetransmitted Polyhedral) plant viruses such as TORSV (Tomato Ringspot Virus), GFLV (Grapevine Fan Leaf Virus) and protein macromolecule from TORSV particles are described. The current response to the applied voltage is measured for eac...

The research of sanitary status and virus presence was conducted on three native Croatian grapevine varieties (Kujudžuša bijela, Zlatarica vrgorska Trnjak crni) that are grown in Dalmatia region. investigation done 492 vines from 31 vineyards. Vines were tested by ELISA (Enzyme-Linked Immunosorbent Assay) four main economic viruses: arabis mosaic (ArMV), fanleaf (GFLV), leafroll-associated 1 3 ...