نتایج جستجو برای: globin

تعداد نتایج: 6744  

2015
Kim Schwarze Abhilasha Singh Thorsten Burmester

Globins are small heme proteins that play an important role in oxygen supply, but may also have other functions. Globins offer a unique opportunity to study the functional evolution of genes and proteins. We have characterized the globin repertoire of two different turtle species: the Chinese softshell turtle (Pelodiscus sinensis) and the western painted turtle (Chrysemys picta bellii). In the ...

2006
Jeremy W. Rupon Shou Zhen Wang Karin Gaensler Joyce Lloyd Gordon D. Ginder

The genes of the vertebrate -globin locus undergo a switch in expression during erythroid development whereby embryonic fetal genes of the cluster are sequentially silenced and adult genes are activated. We describe here a role for DNA methylation and MBD2 in the silencing of the human fetal -globin gene. The -globin gene is reactivated upon treatment with the DNA methyltransferase inhibitor 5-...

Journal: :Blood 2005
Sung-Ho Goh Y Terry Lee Natarajan V Bhanu Margaret C Cam Richard Desper Brian M Martin Ramy Moharram Robert B Gherman Jeffery L Miller

A previously undefined transcript with significant homology to the pseudo-alpha2 region of the alpha-globin locus on human chromosome 16 was detected as part of an effort to better define the transcriptional profiles of human reticulocytes. Cloning and sequencing of that transcript (GenBank AY698022; named mu-globin) revealed an insert with a 423-nucleotide open reading frame. BLASTP and Clusta...

Journal: :Blood 1998
K Ishiguro A C Sartorelli

Using an RNase protection assay, globin mRNA species expressed in clones derived from Ba/F3 and B6SUtA cells transfected with the erythropoietin receptor (EpoR) and selected with erythropoietin (Epo) were compared with globin mRNA species induced in corresponding parental cells by sodium butyrate (SB) and trichostatin A (TSA). betaMajor/betaminor- and -1/-2-globin mRNAs were the major species, ...

Journal: :Blood 2003
Nynke Gillemans Tara McMorrow Rita Tewari Albert W K Wai Carola Burgtorf Dubravka Drabek Nicki Ventress An Langeveld Douglas Higgs Kian Tan-Un Frank Grosveld Sjaak Philipsen

To further our understanding of the regulation of vertebrate globin loci, we have isolated cosmids containing alpha- and beta-globin genes from the pufferfish Fugu rubripes. By DNA fluorescence in situ hybridization (FISH) analysis, we show that Fugu contains 2 distinct hemoglobin loci situated on separate chromosomes. One locus contains only alpha-globin genes (alpha-locus), whereas the other ...

2014
Richard A. Voit Ayal Hendel Shondra M. Pruett-Miller Matthew H. Porteus

Tal-effector nucleases (TALENs) are engineered proteins that can stimulate precise genome editing through specific DNA double-strand breaks. Sickle cell disease and β-thalassemia are common genetic disorders caused by mutations in β-globin, and we engineered a pair of highly active TALENs that induce modification of 54% of human β-globin alleles near the site of the sickle mutation. These TALEN...

2014
Won Ju Yun Yea Woon Kim Yujin Kang Jungbae Lee Ann Dean AeRi Kim

TAL1 is a key hematopoietic transcription factor that binds to regulatory regions of a large cohort of erythroid genes as part of a complex with GATA-1, LMO2 and Ldb1. The complex mediates long-range interaction between the β-globin locus control region (LCR) and active globin genes, and although TAL1 is one of the two DNA-binding complex members, its role is unclear. To explore the role of TAL...

2011
Weiqun Lu Aurelie Mayolle Guoqiang Cui Lei Luo Richard J. Balment

In order to understand the possible role of globin genes in fish salinity adaptation, we report the molecular characterization and expression of all four subunits of haemoglobin, and their response to salinity challenge in flounder. The entire open reading frames of α1-globin and α2-globin genes were 432 and 435 bp long, respectively, whereas the β1-globin and β2-globin genes were both 447 bp. ...

Journal: :Blood 2005
Jie Wen Suming Huang Heather Rogers Liliane A Dickinson Terumi Kohwi-Shigematsu Constance Tom Noguchi

Special AT-rich binding protein 1 (SATB1) nuclear protein, expressed predominantly in T cells, regulates genes through targeting chromatin remodeling during T-cell maturation. Here we show SATB1 family protein induction during early human adult erythroid progenitor cell differentiation concomitant with epsilon-globin expression. Erythroid differentiation of human erythroleukemia K562 cells by h...

Journal: :Human molecular genetics 2002
Patrick A Navas Qiliang Li Kenneth R Peterson Richard A Swank Alex Rohde Julianne Roy George Stamatoyannopoulos

The beta-globin locus control region (LCR) is a powerful regulatory element required for high-level globin gene expression. We have generated transgenic mouse lines carrying a beta-globin locus yeast artificial chromosome lacking the LCR to determine if the LCR is required for globin gene activation. beta-Globin gene expression was analyzed by RNase protection, but no detectable levels of epsil...

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