Various preparations of gamma-globulin homogeneous in the ultracentrifuge showed a similar content of hexose, hexosamine, fucose, and sialic acid. Subfractionation of Fr. II gamma-globulin by zone electrophoresis revealed multiple components of different mean mobilities but containing similar amounts of carbohydrate. Gamma globulin isolated directly from normal serum by zone electrophoresis sho...

Several serum proteins have been shown to be important in modulating leukocyte chemotaxis and inflammation. We investigated the possibility that the multifunctional serum protein Gc-globulin (vitamin D-binding protein) may also enhance the neutrophil chemotactic activity of complement-derived peptides. Purified Gc-globulin by itself did not induce chemotaxis of human neutrophils. However, as li...

T FIAT VITAMIN B12 IN SERUM is l)ound to protein14 has been known since shortly after the isolation of the vitamin. Endogenous vitamin B12 (which may be mainly methyl-Bi2) is bound primarily to an a1-globulin 2.6-10 hut vitamin B12 added to serum in vitro hinds primarily to -globuun (presumably because B12-binding a-globulin is normally almost saturated with endogenous vitamin B12).61#{176} hal...

By ROBERT E. ANDERSON AND Ro L. WALFORD T HE PRESENT study forms part of a continuing effort to develop quantitative methods for detecting antigen-antibody reactions involving nucleated cells as antigen. Here we shall present information regarding the preparation of purified, 1131-labelled anti-human-globulin antibody (AHG antibody) by means of adsorption and elution from normal human globulin,...

Two immunoelectrophoretically defined, heretofore unidentified beta(1)-globulins of human serum, provisionally designated beta(1C)- and beta(1A)-globulin, were isolated by means of preparative electrophoresis and chromatography on anion exchange cellulose. The sedimentation coefficient S(0) (20, w) of beta(1C)-globulin was shown to be 9.5 S, and that of beta(1A)-globulin, 6.9 S. Both proteins w...

When 11-day mice are fed with homologous or heterologous antibodies or with heterologous y-globulin, the maximum concentration of these proteins in their sera is reached 2 h later, irrespective of the amount or specificity of the proteins administered, or of the final con­ centration achieved. The relation between y (= relative titre of antibody, or concentration of heterologous y-globulin, att...

T FIAT VITAMIN B12 IN SERUM is l)ound to protein14 has been known since shortly after the isolation of the vitamin. Endogenous vitamin B12 (which may be mainly methyl-Bi2) is bound primarily to an a1-globulin 2.6-10 hut vitamin B12 added to serum in vitro hinds primarily to -globuun (presumably because B12-binding a-globulin is normally almost saturated with endogenous vitamin B12).61#{176} hal...

T FIAT VITAMIN B12 IN SERUM is l)ound to protein14 has been known since shortly after the isolation of the vitamin. Endogenous vitamin B12 (which may be mainly methyl-Bi2) is bound primarily to an a1-globulin 2.6-10 hut vitamin B12 added to serum in vitro hinds primarily to -globuun (presumably because B12-binding a-globulin is normally almost saturated with endogenous vitamin B12).61#{176} hal...

T FIAT VITAMIN B12 IN SERUM is l)ound to protein14 has been known since shortly after the isolation of the vitamin. Endogenous vitamin B12 (which may be mainly methyl-Bi2) is bound primarily to an a1-globulin 2.6-10 hut vitamin B12 added to serum in vitro hinds primarily to -globuun (presumably because B12-binding a-globulin is normally almost saturated with endogenous vitamin B12).61#{176} hal...