A novel FAD-dependent glucose dehydrogenase (FAD-GDH) was found and its enzymatic property for glucose sensing was characterized. FAD-GDH oxidized glucose in the presence of some artificial electron acceptors, except for O2, and exhibited thermostability, high substrate specificity and a large Michaelis constant for glucose. FAD-GDH was applied to an amperometric glucose sensor with Fe(CN)6(3-)...

The present study investigated the relationship between blood and subcutaneous interstitial fluid (ISF) glucose by employing an amperometric glucose sensor specifically developed for 3-day continuous glucose monitoring. The apparent sensor sensitivity and ISF glucose equilibration delay were estimated on separate days during hyperglycemic clamps in four dogs in which insulin was either suppress...

The potential use of hydrazine sulfate was examined for the catalytic reduction of enzymatically generated H2O2 in a biosensor system. The performance of the hydrazine-based sensor was compared with an HRP-based glucose sensor as a model of a biosensor. Hydrazine and HRP were covalently immobilized onto a conducting polymer layer with glucose oxidase. The direct electron transfer reactions of t...

BACKGROUND Hydrogels alone and in combination with microsphere drug delivery systems are being considered as biocompatible coatings for implantable glucose biosensors to prevent/minimize the foreign body response. Previously, our group has demonstrated that continuous release of dexamethasone from poly(lactic-co-glycolic acid) (PLGA) microsphere/poly(vinyl alcohol) (PVA) hydrogel composites can...

OBJECTIVE To characterize glucose levels during daily living using continuous glucose monitors (CGMs) in nondiabetic individuals. RESEARCH DESIGN AND METHODS Seventy-four healthy children, adolescents, and adults aged 9-65 years with normal glucose tolerance used a blinded CGM device for 3 to 7 days. RESULTS Sensor glucose concentrations were 71-120 mg/dl for 91% of the day. Sensor values w...

For fifty years, tremendous efforts have been directed towards the development of glucose sensors for tight glycemic control of diabetic patients. Today, millions of diabetics test their blood glucose level daily, making glucose the most commonly tested analyte. Recently, subcutaneous implantable needle-type sensors became commercially available for continuous glucose monitoring. However, these...

We present a MEMS affinity glucose sensor using permittivity measurements. This device employs a biocompatible glucose-specific polymer as the sensing solution. By introducing the polymer solution between two electrodes, the permittivity of the polymer solution can be measured from the capacitance changes induced by glucose binding. We characterize the frequency-dependent complex permittivity a...

We present a viscometric affinity biosensor for continuous monitoring of glucose in biological fluids such as blood and plasma. The sensing principle of this chemico-mechanical sensor is based upon the viscosity variation of a sensitive fluid with glucose concentration. Basically, this device includes both an actuating and a sensing piezoelectric diaphragms as well as a flow-resistive microchan...