Protein translation is an energetically demanding process that must be regulated in response to changes in nutrient availability. Herein, we report that intracellular methionine and cysteine availability directly controls the thiolation status of wobble-uridine (U34) nucleotides present on lysine, glutamine, or glutamate tRNAs to regulate cellular translational capacity and metabolic homeostasi...

1. Aspergillus nidulans, Neurospora crassa and Escherichia coli were grown on media containing a range of concentrations of nitrate, or ammonia, or urea, or l-glutamate, or l-glutamine as the sole source of nitrogen and the glutamate dehydrogenate and glutamine synthetase of the cells measured. 2. Aspergillus, Neurospora and Escherichia coli cells, grown on l-glutamate or on high concentrations...

The optimal positioning of salt-bridging interactions in a parallel alpha-helical homotrimeric coiled coil has been explored in a metal ion-assembled polypeptide trimer of 60 residues; arginine-glutamate pairs are more stabilizing than the corresponding lysine-glutamate pairs, and optimal stabilization is obtained with positively charged arginine residues at the c positions of the alpha-helical...

Behcet's disease (BD) with arthritis is often confused with seronegative arthritis (SNA) because of shared clinical symptoms and the lack of definitive biomarkers for BD. To investigate possible metabolic patterns and potential biomarkers of BD with arthritis, metabolomic profiling of synovial fluid (SF) from 6 patients with BD with arthritis and 18 patients with SNA was performed using gas chr...

The transport of L-proline, L-lysine and L-glutamate in rat red blood cells has been studied. L-proline and L-lysine uptake were Na(+)-independent. When the concentration dependence was studied both showed a non-saturable uptake assimilable to a difussion-like process, with high Kd values (0.718 and 0.191 min-1 for L-proline and L-lysine respectively). Rat red blood cells showed high impermeabi...

By kinetic studies and the identification of the labeled peptide of the [WIKNCO-modified protein, the inactivation of bovine liver glutamate dehydrogenase by potassium cyanate can be attributed to the carbamylation of the e-amino group of lysine-97. Although the e-amino group of lysine-97 is the major site of carbamylation by cyanate, the oc-amino group of alanine-l and E-amino group of lysine-...

A 4-wk experiment was conducted to compare the effect of feeding crystalline lysine relative to the lysine from soybean meal (SBM) on growth performance, body chemical composition, and tissue deposition rates in nursery pigs. Thirty-six pigs were used [18 barrows and 18 gilts, 15 d old; initial BW (mean ± SEM) = 6.11 ± 0.11 kg]. Six pigs (3 barrows and 3 gilts) were killed at the beginning of t...

An 11-week feeding trial was conducted to assess the dietary lysine requirement for juvenile Hemibagrus wyckioides. Six isoproteic and isoenergetic diets (41.66% crude protein, 20.56 kJ/g gross energy) containing 1.1%, 1.5%, 1.9%, 2.3%, 2.7%, 3.1% were prepared feed juveniles (average initial body weights of 1.25 g), respectively. The weight gain, specific growth rate (SGR), conversion ratio (F...

The influence on protein accretion and whole-body protein turnover of changing dietary protein quality while maintaining constant energy intake was studied by varying the degree of lysine supplementation of a lysine-deficient barley-based diet given to growing pigs. Measurements of nitrogen metabolism and whole-body protein turnover, using both classical and 15N end-product methods following a ...