We evaluated glutathione transferase (GST) activities and the levels of glutathionylated hemoglobin in the RBC of 42 workers exposed to 1,3-butadiene in a petrochemical plant, using 43 workers not exposed to 1,3-butadiene and 82 foresters as internal and external controls, respectively. Median 1,3-butadiene exposure levels were 1.5, 0.4, and 0.1 microg/m3 in 1,3-butadiene-exposed workers, in wo...

* Correspondence: [email protected]

We demonstrate an approach to automatically generating kinetic models of metabolic networks. In a first step, the metabolic network is characterised by its stoichiometric structure. Then to each reaction a kinetic equation is associated describing the metabolic flux. For the kinetics we use a formula that is universally applicable to reactions with arbitrary numbers of substrates and products. ...

The application of two-dimensional J-resolved nuclear magnetic resonance spectroscopy to determine the structure of two bile metabolites isolated from rats injected interperitoneally with bromobenzene is described. The structures of the two molecules are obtained unambiguously from the proton-proton spin coupling constants. This paper discusses the fundamentals of the technique and demonstrates...

Glutathione transferase A has been purified from rat liver. The enzyme catalyzes the conjugation of glutathione with compounds bearing an electrophilic site, especially those in which the electrophilic site is on, or cr to, an aromatic ring. The enzyme has a molecular weight of 45,000 and is composed of two similar subunits. Initial velocity, product inhibition, and binding studies indicate a b...

1 The Two-Hybrid System: A Personal View Stanley Fields and Paul L. Bartel .............................................. 3 2 Growth and Maintenance of Yeast Lawrence W. Bergman ................................................................ 9 3 Media Formulations for Various Two-Hybrid Systems Michael Saghbini, Denise Hoekstra, and Jim Gautsch ........ 15 4 Yeast Two-Hybrid Vectors and Strain...

Background: Glutathione S-transferases (GSTs) are detoxifying enzymes that contribute to the glutathione-ascorbic acid (vitamin C) antioxidant cycle. Objective: The objective was to determine whether GST genotypes modify the association between dietary vitamin C and serum ascor-